林业科学2013，Vol.49Issue(6)：60-67,8.DOI:10.11707/j.1001-7488.20130609
外源NO对花楸树胚胎萌发和幼苗发育初期活性氧积累的影响
Effects of Exogenous Nitric Oxide on Embryo Germination and ROS Accumulation in Seedling Growth Initial Stage of Sorbus pohuashanensis
摘要
Abstract
In this paper,mature zygotic embryo of Sorbus pohuashanensis were used to study effects of an exogenous nitric oxide donor (sodium nitroprusside,SNP),NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-l-oxyl-3-oxide (PTIO),abscisic acid (ABA) and 2,5-norbornadiene (NBD)-an inhibitor of the ethylene receptor on the embryo germination and reactive oxygen species (ROS) accumulation at early stage of the seedling development by the petri dish germination method indoors.The results showed that the inhibitory effect of PTIO or ABA on the embryo germination was relieved by the SNP pretreatment under light.However,the SNP pretreatment did not influence the inhibitory action of NBD to embryo germination.The SNP pretreatment increased the chlorophyll content in leaves of seedlings,which was not removed by PTIO treatment.The contents of hydrogen peroxide (H2O2) and superoxide anion in seedling leaves were increased by SNP pretreatment,which was cancelled by PTIO treatment.The PTIO treatment reduced the superoxide anion content in seedling roots,which was removed by SNP pretreatment.The results would lay the foundation for interpreting the relationships between reactive nitrogen species (RNS) mediated NO-dependent) dormancy removal and ROS (H2O2 and superoxide anion) accumulation in the embryo germination.关键词
花楸树/胚胎萌发/一氧化氮/脱落酸/乙烯Key words
Sorbus pohuashanensis / embryo germination / nitric oxide / abscisic acid / ethylene分类
农业科技引用本文复制引用
杨玲,王佳男,边磊,沈海龙..外源NO对花楸树胚胎萌发和幼苗发育初期活性氧积累的影响[J].林业科学,2013,49(6):60-67,8.基金项目
黑龙江省教育厅科学技术指导项目(11553034) （11553034）
中央高校基本科研业务费专项资金(DL10BA04). （DL10BA04）
