林业科学2013，Vol.49Issue(8)：35-42,8.DOI:10.11707/j.1001-7488.20130806
内源激素IAA和ABA含量与黑杨苗期高生长关系
Correlation between Endogenous IAA, ABA Contents and Height Growth of Black Poplar at the Seedling Stage
摘要
Abstract
The contents of endogenous IAA and ABA in different tissues at the seedling stage of three black poplar clones were determined by GC-MS to investigate correlation between the height growth of poplar and the endogenous hormones,and to identify influence factors of early selection in poplar seedling height.At the same time,height growth of the seedling was measured,and tissue structure of the elongating internode and mature internode were discriminated by paraffin section.The results showed that:1) Increase in monthly internode numbers was significantly positively correlated with the monthly seedling height increment.There were no significant correlation between the total length of the first internode and the second internode and seedling monthly increasing height,IAA content,ABA content,the ratio of IAA to ABA.2) The cells of elongating internode were small and arranged compactly,and the secondary xylem was not distinct.The cells of mature internode were bigger and arranged loosely,and the secondary xylem was distinct.3) the monthly increasing internode numbers,monthly increasing seedling height,IAA content,ABA content and the ratio of IAA to ABA of three young tissues of the clone ‘ Lingfeng1 ' increased from May to July.In July,sequence of monthly increasing internode number and monthly increasing seedling height were not correlated with endogenous IAA content,and were significantly negatively correlated with endogenous ABA contents of young leaves,and were significantly positively correlated with the ratio of IAA to ABA of apical buds.Endogenous IAA content of young leaves and other organs could not be used as the index to predict sequence of seedling height of clones.But Endogenous ABA content of young leaves was able to be used as the reference index.Early prediction of seedling height in poplar can be conducted by using the ratio of IAA to ABA of apical buds.This study lays the groundwork for early selection of prior clones in seedling height and the basis of genetic engineering breeding for IAA and ABA.关键词
生长素/脱落酸/杨树/高生长/早期预测/气-质联用/石蜡切片Key words
IAA/ ABA/ poplar/ height growth/ predict early/ GC-MS/ paraffin section分类
农业科技引用本文复制引用
杨成超,黄秦军,苏晓华..内源激素IAA和ABA含量与黑杨苗期高生长关系[J].林业科学,2013,49(8):35-42,8.基金项目
国家"十二五"科技支撑计划(2012BAD01B03). （2012BAD01B03）
