生态与农村环境学报2013，Vol.29Issue(4)：438-442,5.
浮游植物叶绿素a浓度测定方法的比较研究
Comparison of Methods for Determination of Phytoplankton Chlorophyll-a
摘要
Abstract
As chlorophyll-a (Chl-a) is used to characterize phytoplankton biomass,its content in a waterbody is commonly used as an indicator of eutrophication level of the waterbody.Although there are a number of measurement procedures currently in use,not a unified standard method is developed.A retrospect is presented of the development history of the methods for determination of Chl-a.On such a basis,a comparative study was done of the methods in cell discruption technique and type of extractant.By comparing advantages and disadvantages of the various determination methods,a relatively effective method,hot-ethanol method,is proposed.This method is easy to operate,high in extracting rate,stable,reliable and harmless to human health.Besides,a brief introduction is presented the three commonly-used determination methods,i.e.spectrofluorometry,high efficiency liquid chromatographic and spectrophotometry,and their respective procedures,applicabilities,advantages and disadvantages.The comparison demonstrates that the combination of the hot ethanol extraction method with the spectrophotometry is a good method,worth extrapolating.It is expected that the review may provide some bases for development of a standard method for determination of Chl-a concentration in China.关键词
叶绿素a/提取液/热乙醇法/分光光度法Key words
chlorophyll-a/ extracting solvent/ hot-ethanol extraction/ spectrophotometry分类
资源环境引用本文复制引用
徐彩平,刘霞,陈宇炜..浮游植物叶绿素a浓度测定方法的比较研究[J].生态与农村环境学报,2013,29(4):438-442,5.基金项目
国家重点基础研究发展计划(2012CB417005) （2012CB417005）
