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镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响

刘耀明 杨慧敏 张育平 吴海花 张建珍 马恩波 郭亚平

农业环境科学学报2013,Vol.32Issue(7):1321-1327,7.
农业环境科学学报2013,Vol.32Issue(7):1321-1327,7.DOI:10.11654/jaes.2013.07.006

镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响

Acute Effects of Cd2+ and Cr6+ on Detoxification Enzymes and Polyphenol Oxidase in Oxya chinensis

刘耀明 1杨慧敏 1张育平 1吴海花 2张建珍 1马恩波 1郭亚平1

作者信息

  • 1. 山西大学应用生物学研究所,太原030006
  • 2. 太原师范学院生物系,太原030031
  • 折叠

摘要

Abstract

Acute effects of Cd2+ and Cr6+ on detoxification enzymes and polyphenol oxidase(CarE,GST,ACP,AKP and PPO) in 5th instar nymphs of Oxya chinensis were studied under laboratory control condition.The results showed that CarE activities were activated after insects were exposed to the highest concentration of Cd2+(7.08 mg· kg-1) for 48 h and Cr6+(3.40 mg· kg-1) for 72 h,respectively.ACP activity was inhibited by Cd2+ at 96 h.AKP activity increased-in exposed to Cr6+ for 72 h.However,ACP activity increased at the lower concentration of 1.80 mg·kg-1 Cr6+ and declined at the higher concentration of 3.40 mg·kg-1 Cr6+ at 24 h and 48 h.GST activites were increased by Cd2+ at 48 h and by Cr6+ at 72 h and 96 h,respectively.PPO activities were decreased by Cd2+ at 24 h and by Cr6+ at 96 h,respectively.These results indicated that detoxification enzymes and polyphenol oxidase activity changed in response to toxic effects of heavy metal (Cd and Cr),while the changes were related with the exposed dose and time of heavy metal.

关键词

//代谢酶/多酚氧化酶/中华稻蝗

Key words

Cd/ Cr/ detoxification enzyme/ polyphenol oxidase/ Oxya chinensis

分类

资源环境

引用本文复制引用

刘耀明,杨慧敏,张育平,吴海花,张建珍,马恩波,郭亚平..镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响[J].农业环境科学学报,2013,32(7):1321-1327,7.

基金项目

国家自然科学基金项目(31071980) (31071980)

山西省自然科学基金项目(2011011033-1) (2011011033-1)

农业环境科学学报

OA北大核心CSCDCSTPCD

1672-2043

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