农业环境科学学报2013,Vol.32Issue(7):1321-1327,7.DOI:10.11654/jaes.2013.07.006
镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响
Acute Effects of Cd2+ and Cr6+ on Detoxification Enzymes and Polyphenol Oxidase in Oxya chinensis
摘要
Abstract
Acute effects of Cd2+ and Cr6+ on detoxification enzymes and polyphenol oxidase(CarE,GST,ACP,AKP and PPO) in 5th instar nymphs of Oxya chinensis were studied under laboratory control condition.The results showed that CarE activities were activated after insects were exposed to the highest concentration of Cd2+(7.08 mg· kg-1) for 48 h and Cr6+(3.40 mg· kg-1) for 72 h,respectively.ACP activity was inhibited by Cd2+ at 96 h.AKP activity increased-in exposed to Cr6+ for 72 h.However,ACP activity increased at the lower concentration of 1.80 mg·kg-1 Cr6+ and declined at the higher concentration of 3.40 mg·kg-1 Cr6+ at 24 h and 48 h.GST activites were increased by Cd2+ at 48 h and by Cr6+ at 72 h and 96 h,respectively.PPO activities were decreased by Cd2+ at 24 h and by Cr6+ at 96 h,respectively.These results indicated that detoxification enzymes and polyphenol oxidase activity changed in response to toxic effects of heavy metal (Cd and Cr),while the changes were related with the exposed dose and time of heavy metal.关键词
镉/铬/代谢酶/多酚氧化酶/中华稻蝗Key words
Cd/ Cr/ detoxification enzyme/ polyphenol oxidase/ Oxya chinensis分类
资源环境引用本文复制引用
刘耀明,杨慧敏,张育平,吴海花,张建珍,马恩波,郭亚平..镉和铬急性染毒对中华稻蝗解毒酶及多酚氧化酶的影响[J].农业环境科学学报,2013,32(7):1321-1327,7.基金项目
国家自然科学基金项目(31071980) (31071980)
山西省自然科学基金项目(2011011033-1) (2011011033-1)