皮革科学与工程2013，Vol.23Issue(4)：23-27,5.
铬革屑的酶解研究及其产物氨基酸成分分析
Hydrolysis of Chrome Shavings with Protease and Analyses the Amino Acid Component of the Products
简未平 1王全杰1
作者信息
- 1. 大连工业大学轻工与化学工程学院,辽宁大连116034
- 折叠
摘要
Abstract
Optimum conditions of hydrolysis on chrome shavings with protease were investigated based on the hydrolysis rate.Some influence factors such as the hydrolytic temperature,hydrolytic time,the amount of protease,and hydrolytic pH were determined.The optimum conditions were as follows:hydrolytic temperature 55 ℃,hydrolytic time 4h,the amount of protease 2％,hydrolytic pH=7.0.The hydrolysis rate of the chrome shavings can be increased to 97.61％ under such conditions,nitrogen yield is 78.84％,and the chrome contents of the hydrolysate is only 0.31 ×10-4g/L.This paper also analysed the free amino acid component of the hydrolysate through High Performance Liquid Chromatography.关键词
中性蛋白酶/水解/铬革屑/水解率/高效液相色谱Key words
neutral enzyme/ hydrolysis/ chrome shavings/ hydrolysis rate/ high performance liquid chromatography分类
轻工纺织引用本文复制引用
简未平,王全杰..铬革屑的酶解研究及其产物氨基酸成分分析[J].皮革科学与工程,2013,23(4):23-27,5.
