热带作物学报2013，Vol.34Issue(5)：795-799,5.DOI:10.3969/j.issn.1000-2561.2013.05.001
巴西橡胶树肌动蛋白的原核表达及鉴定
Prokaryotic Expression and Identification of Actin Protein in Laticifer Cells of Rubber Tree(Hevea brasiliensis Müll.Arg.)
摘要
Abstract
ACTIN,a component of cytoskeleton,is one of the constituents of the plugs at the end of the severed laticifers.In order to study the interaction of ACTIN with proteins in the plugs,the A CTIN gene prokaryotic expression vector was successfully constructed.Recombinant protein was expressed in abundance in the E.coil BL21 (DE3) after being induced by 0.1 mmol/L IPTG for 2 hours at 37 ℃.The recombinant protein with a molecular mass of about 41.7 ku was mainly present as inclusion body.It was further purified by Ni-NTA affinity chromatography and identified by western-blot with mouse anti-HIS tagged monoclonal antibody.This study laid a good foundation of revealing the mechanism for plug formation at the end of severed laticifers.关键词
巴西橡胶树/肌动蛋白细胞骨架/乳管堵塞/原核表达Key words
Hevea brasiliensis Miüll.Arg./ Actin cytoskeleton/ Laticifer plugging/ Prokaryotic expression分类
农业科技引用本文复制引用
邓顺楠,史敏晶,吴绍华,田维敏..巴西橡胶树肌动蛋白的原核表达及鉴定[J].热带作物学报,2013,34(5):795-799,5.基金项目
国家自然科学基金项目(No.30170535) （No.30170535）
现代农业产业技术体系建设专项(No.CARS-34-GW1). （No.CARS-34-GW1）
