热带作物学报2013，Vol.34Issue(5)：800-806,7.DOI:10.3969/j.issn.1000-2561.2013.05.002
橡胶树HbFCA启动子的克隆及其在橡胶树中的表达分析
Cloning and Expression Analysis of the Promoter to HbFCA in Hevea brasiliensis
摘要
Abstract
Flowering control locus A (FCA),as a component of the autonomous pathway,is involved in regulating the flowering time in different developmental stage of plant.The promoter of HbFCA of 28 cis-regulation elements with mainly cis-acting element in promoter and enhancer regions (TAAT-box and CAAT-box) and light responsive element was cloned by TAIL-PCR.And GUS gene was strongly expressed in the leaves,roots and somatic embryo in different developmental stage and weakly in the stems in the rubber tree transgenic plant with PHbFCA:GUS.It is indicated that HbFCA plays important role in different developmental stage of rubber tree,and the transgenic plant with PHbFCA:GUS was helpful for the analysis of HbFCA functions.关键词
橡胶树/FCA (Flowering control locus A)/启动子/转基因Key words
Hevea brasiliensis/ FCA (Flowering control locus A)/ Promoter/ Genetic transformation分类
农业科技引用本文复制引用
华玉伟,孙芳,黄天带,蔡海滨,杨加伟,黄华孙..橡胶树HbFCA启动子的克隆及其在橡胶树中的表达分析[J].热带作物学报,2013,34(5):800-806,7.基金项目
国家天然橡胶产业技术体系(No.nycytx-34)和国家自然科学基金(No.31200503). （No.nycytx-34）
