热带作物学报2013，Vol.34Issue(5)：807-814,8.DOI:10.3969/j.issn.1000-2561.2013.05.003
橡胶树5-烯醇式丙酮莽草酸-3-磷酸合成酶基因的克隆及其响应非生物胁迫的表达分析
Cloning and Expression Analysis of 5-Enolpyruvylshikimate-3-phosphate Synthetase Gene Under Abiotic Stress in Hevea brasiliensis
摘要
Abstract
5-Enolpyruvylshikimate-3-phosphate (EPSPs) synthase is an important enzyme of the shikimate pathway.It is associated with the synthesis of a lot of secondary metabolites related to stress tolerance.In this study,a 2 025 bp HbEPSPS cDNA,containing a 1572 bp ORF and encoding 523 amino acids,was cloned from CATAS-73397 by 5＇ and 3＇ RACE.Bioinformatics analysis showed that the molecular weight of HbEPSPS protein was 56.01 u and its isoelectric point was 7.91.The full length of HfbEPSPS gene was 4600 bp,and there were eight exons and seven introns in HbEPSPS gene.The results of qRT-PCR showed that HbEPSPS could express in leaves,bark,flower,latex and embryo,and among these tissues,but the highest expression level of HbEPSPS was in leaves.The expression of HbEPSPS could be induced by hormones,drought,salt and low temperature,and it was significantly up-regulated under MeJA,ETH and PEG stress,respectively.The cloning and expression analysis of HbEPSPS gene under abiotic stress will provide theoretical basis for rubber tree in the abiotic stress research.关键词
橡胶树/EPSPS/非生物胁迫/莽草酸途径Key words
Hevea brasiliensis/ EPSPS/ Abiotic stress/ Shikimate pathway分类
农业科技引用本文复制引用
李雅超,安泽伟,谢黎黎,翟琪麟,黄华孙..橡胶树5-烯醇式丙酮莽草酸-3-磷酸合成酶基因的克隆及其响应非生物胁迫的表达分析[J].热带作物学报,2013,34(5):807-814,8.基金项目
国家天然橡胶产业技术体系(No.CARS-34) （No.CARS-34）
国家自然科学基金项目(No.30960319、No.31271796) （No.30960319、No.31271796）
中国热带农业科学院橡胶研究所基本科研业务费专项(No.1630022011002). （No.1630022011002）
