热带作物学报2013，Vol.34Issue(5)：866-869,4.DOI:10.3969/j.issn.1000-2561.2013.05.012
橡胶树体胚遗传转化中早代转化胚状体的PCR鉴定
The PCR Analysis of Early Embryos in Hevea Somatic Embryogenesis Transformation
摘要
Abstract
Agrobacterium tumefaciens-mediated Hevea somatic embryogenesis transformation system was successfully established.But a large propagation efficiently propagated from the secondary embryogenesis would lead to heavy burden in the following subculture.Thus the analysis of early positive embryo clones is very important.In this paper,the method of DNA extraction was optimized according to the conventional method of CTAB,meanwhile,the suitable weight of embryo used for PCR analysis was chosen.Experiments indicated that the optimized DNA extraction method and the scale of 1～3 mg all could accord with the PCR analysis of early embryos.Moreover,the results of PCR analysis were coincided with GUS histochemical staining.Hence,the method in this paper is available to early embryos screen in somatic embryogenesis transformation.关键词
橡胶树/遗传转化/次生体胚/PCR/GUSKey words
Rubber tree/ Genetic transformation/ Secondary embryo/ PCR/ GUS分类
农业科技引用本文复制引用
李季,黄天带,蔡海滨,华玉伟,黄华孙..橡胶树体胚遗传转化中早代转化胚状体的PCR鉴定[J].热带作物学报,2013,34(5):866-869,4.基金项目
国家自然科学基金(No.30960322) （No.30960322）
国家自然科学基金(No.31200503) （No.31200503）
现代农业产业技术体系建设专项(No.NYCYTX-34). （No.NYCYTX-34）
