热带作物学报2013，Vol.34Issue(5)：899-904,6.DOI:10.3969/j.issn.1000-2561.2013.05.017
黑美人粗肋草的组织培养研究
Tissue Culture and Rapid Propagation of Aglaonema commutatum Schott cv Treubii
摘要
Abstract
Taken the stem segments with side buds of Aglaonema commutatum Schott cv Treubii as explants,the effects of different sterilization methods,inoculation methods,and different concentrations of plant growth regulators in medium on in vitro rapid propagation of A.commutatum were studied.The results showed that:(1) the more suitable sterilization method for in vitro rapid propagation of A.commutatum was wiping the explants with 75％ alcohol in combination with treating the explants with 0.1％ HgCl2 for 20 min; (2) the more suitable inoculation method for adventitious buds induction was inoculating the explants into culture medium horizontally; (3) the most suitable culture medium for adventitious buds induction was MS+2.0 mg/L 6-BA+0.1 mg/L NAA,and under this culture medium the induction rate of 94.10％ was achieved; (4) the more appropriate medium for multiplication was MS+4.0 mg/L 6-BA+0.05 mg/L NAA,and under this culture medium the multiplication coefficient of 6.30 was achieved and the most suitable generation numbers for proliferation culture was from five to six; (5) the more suitable culture medium for rooting culture was 1/2MS+0.2～0.3 mg/L NAA,and under this culture medium the rooting rate of 100％ was achieved; (6) and the survival rate was higher when transplanting to peat or garden soil medium under temperature and humid preservation condition.关键词
黑美人粗肋草/茎段/组织培养/快速繁殖Key words
Aglaonema commutatum Schott cv Treubii/ Stem/ Tissue culture/ Rapid propagation分类
农业科技引用本文复制引用
刘俊仙,熊发前,于文进,李松,刘丽敏,刘红坚,杨柳,方锋学,龙明华..黑美人粗肋草的组织培养研究[J].热带作物学报,2013,34(5):899-904,6.基金项目
国家“十一五”科技支撑计划项目(No.2007BAD68B04) （No.2007BAD68B04）
国家自然科学基金项目(No.31240059) （No.31240059）
广西农业科学院科技发展基金青年科技专项(桂农科2013JQ03). （桂农科2013JQ03）
