热带作物学报2013,Vol.34Issue(7):1252-1256,5.DOI:10.3969/j.issn.1000-2561.2013.07.010
石斛兰亲缘关系的SSR分析
Analysis of Genetic Relationship of Ornamental Dendrobium by SSR Marker
摘要
Abstract
The genetic diversity analysis of ornamental Dendrobium is significant in the utilizztion of the germplasm resources.The genomic DNA of 28 popular commercial Dendrobium hybrids were tested with SSR using 11 primer pairs which were screened from 22 primer pairs.A total of 158 polymorphic alleles were obtained,with an average of 14 for each primer pairs.The SSR based genetic similarity ranged from 0.04 to 0.85.The hybrids could be divided 28 hybrids into five groups at 0.295 level similarity coefficient using UPGMA cluster analysis based on SSR molecular markers data.The results of cluster analysis showed that there was a rich genetic diversity and a broad genetic variation in Dendrobium cultivars.The SSR approach was more efficient in molecular analysis of genetic background and relationship of Dendrobium cultivars.关键词
石斛兰/SSR/亲缘关系/遗传多样性Key words
Ornamental Dendrobium/ SSR/ Genetic relationship/ Genetic diversity分类
生物科学引用本文复制引用
任羽,王呈丹,徐世松,黄少华,张银东..石斛兰亲缘关系的SSR分析[J].热带作物学报,2013,34(7):1252-1256,5.基金项目
海南省自然科学基金项目(No.312024) (No.312024)
国家科技支撑计划项目(No.2007BAD45B06). (No.2007BAD45B06)