四川医学2013，Vol.34Issue(4)：445-447,3.
熊果苷对A375与HACAT共培养模型中黑素细胞酪氨酸酶活性的影响
Effect of Arbutin on the in vitro model of the melanoma cells and keratinocyte co-culture system
摘要
Abstract
Objective To establish the in vitro co-culture model of melanoma cells and keratinocytes,and observe the effect of Arbutin on pigmentation in co-culture model of melanoma cells and keratinocytes.Methods We cultivated the melanoma cells and keratinocytes respectively and then constructed the mixed cultivating model of them.After arbutin was added to the model,celludar proliferation,tyrosinase activity and melanin content were measured by MTT assay,L-DOPA oxidation assay and NaOH assay respectively.Results The in vitro co-culture model of melanoma cells and keratinocytes was established successfully The proliferation of co-culture cells,tyrosinase activity and the melanin synthesis were markedly suppressed by arbutin in a dose-dependent manner.The significant suppression was observed with 1,0.5,0.2mg/ml of arbutin than that with control.Conclusion We have successfully constructed the co-culture model of melanoma cells and keratinocytes,and the results indicate that Arbutin had a strong inhibition on celludar proliferation,tyrosinase activity and melanin content in a dose-dependent manner.关键词
共培养模型/黑素瘤细胞/角质形成细胞/熊果苷Key words
co-culture model/melanoma cells/keratinocytes/Arbutin分类
医药卫生引用本文复制引用
陈凤江,郭云辉,陈巧云..熊果苷对A375与HACAT共培养模型中黑素细胞酪氨酸酶活性的影响[J].四川医学,2013,34(4):445-447,3.基金项目
黑龙江省自然科学基金面上项目(编号:D200810) （编号:D200810）
