四川中医2013，Vol.31Issue(7)：42-45,4.
益水生新饮对自发性高血压大鼠TGF-β1 CTGF FN信号通路的影响
The effect of Yishui Shengxin Decoction on TGF-β1, CTGF, FN Signal Pathways in Spontaneously Hypertensive Rats (SHR)
摘要
Abstract
Objective:To study the effect of Yishui Shengxin Decoction on TGF-β1,CTGF and FN signal pathways in kidney tissue of 12 weeks spontaneously hypertensive rats (SHR).Revealing how did Yishui Shengxin Decoction protect kidney tissue of SHR and its function targets.Methods:Fifty 12 weeks male SHR were randomly divided into five groups:Yishui Shengxin Decoction low-dose group [35.55 g/ (kg.d),low-dose group for short],Yishui Shengxin Decoction middle-dose group [71.10g/ (kg.d),middle-dose group for short],Yishui Shengxin Decoction high-dose group [14.20g/ (kg.d),highdose group for short],Benazepril group [10mg/ (kg · d)],model group,ten for each group,intragastric administration of physiological saline for model group,once a day,eight weeks in all.Put these SHR rats to death after eight weeks.Testing TGF -β1,CTGF,FN mRNA and protein in kidney tissue of each SHR.Observing the changes of renal pathology and blood pressure in each group.Results:(1) Compared with the model group,blood pressure in Yishui Shengxin Decoction group and Benazepril group had a significant decrease (P＜0.01),compared high-dose group with Benazepril group,there was a significant difference (P＜0.01).(2) Pathological examination showed:compared with Yishui Shengxin Decoction group and Benazepril group,affections of intercapillary cells and ground substance hyperplasia obviously speeded up in model group.(3) Compared with the model group,expressions of TGF-β1,CTGF and FN in kidney tissue of Yishui Shengxin Decoction group and Benazepril group reduced significantly (P＜0.05,P＜0.0l),compared high-dose group with Benazepril group,down-regulation of CTGF have a significant difference (P＜0.01).Conclusions:Yishui Shengxin Decoction can reduce SHR rats early renal glomerulosclerosis and renal interstitial fibrosis,mainly through suppression expression of TGF-β1,CTGF and FN signal pathways in kidney,so it plays a role in delaying the progress of hypertension and protecting kidney.关键词
益水生新饮/自发性高血压大鼠/生长转化因子-β1(TGF-β1)/结缔组织生长因子(CTGF)/桥连接蛋白(FN)Key words
Yishui Shengxin Decoction/ Spontaneously hypertensive rats/ Transforming growth factor-β1 (TGF-β1) /Connective tissue growth factor (CTGF) / Fibronection (FN)分类
医药卫生引用本文复制引用
徐宏,韦丽丽,关建国,郭婧姣,赵先锋..益水生新饮对自发性高血压大鼠TGF-β1 CTGF FN信号通路的影响[J].四川中医,2013,31(7):42-45,4.基金项目
广西自然科学基金资助项目(编号:2011GXNSFA018218),广西中医药管理局中医药科技专项资助项目(编号:GZYZ1122),柳州市"新世纪个十百人人才工程"专项资金资助项目. （编号:2011GXNSFA018218）
