山东医药2013，Vol.53Issue(28)：13-16,后插1,5.DOI:10.3969/j.issn.1002-266X.2013.28.004
磁性纳米顺铂微球联合磁流体热疗对卵巢癌skov-3细胞增殖、凋亡及侵袭的影响
Effects of magnetic nano-cisplatin microspheres combined with magnetic fluid hyperthermia on the proliferation, apoptosis and invasion of skov-3 cell line of ovarian cancer
摘要
Abstract
Objective To investigate the effects of magnetic nano-cisplatin microspheres combined with magnetic fluid hyperthermia on the proliferation,apoptosis and invasion of skov-3 cell line of ovarian cancer and the mechanism.Methods The logarithmic growth phase skov-3 cells were divided into five groups:the control group,naked drug group (cisplatin 5μmol/L),nano drug group (cisplatin 5 μmol/L + Fe304 magnetic nano-cisplatin microspheres lg/L),magnetic thermochemotherapy group (Fe304 magnetic nano-cisplatin microspheres 1 g/L) and magnetic heating group (Fe304 magnetic nanoparticles 1 g/L).Cells in the control group,naked drug group and nano drug group were cultured in the incubator for 24 h.Cells in the magnetic thermochemotherapy group and magnetic heating group were treated by magnetic fluid hyperthermia under the electromagnetic field for 24 h.The proliferation was measured by MTT; the levels of CD44v6,MMP-2 mRNA expression were measured by RT-PCR; the invasive ability of cells was detected by transwell test; and the apoptotic rate was detected by flow cytometry (FCM).Results Compared with the control group,inhibitory rate of cell proliferation,apoptosis rate and invasion inhibition rate in the remaining 4 groups were increased (all P ＜ 0.05).There were no statistical significant differences in the expression of MMP-2,CD44v6 mRNA between nano drug group and control group (P ＞0.05).MMP-2,CD44v6 mRNA expression of magnetic heating group,naked drug group and magnetic thermochemotherapy group was decreased gradually (P ＜ 0.05 or P ＜ 0.01).There were statistically significant differences in MMP-2 and CD44v6 mRNA expression between the nano drug group,magnetic heating group,naked drug group and magnetic thermoche-motherapy group (all P ＜ 0.05).Conclusion Magnetic nano-cisplatin microspheres combined with magnetic fluid hyperthermia can not only enhance the inhibitory effects of proliferation and invasion,but also induce the apoptosis,which is associated with the down-regulation of MMP-2,CD44v6 mRNA expression.关键词
卵巢肿瘤/磁性纳米顺铂微球/磁流体热疗/CD44v6/基质金属蛋白酶2Key words
ovarian neoplasms / magnetic nano-cisplatin microspheres/ magnetic fluid hyperthermia/ CD44v6 / matrix metalloproteinase 2分类
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徐云钊,奚庆华,张玉泉..磁性纳米顺铂微球联合磁流体热疗对卵巢癌skov-3细胞增殖、凋亡及侵袭的影响[J].山东医药,2013,53(28):13-16,后插1,5.
