山西医科大学学报2013，Vol.44Issue(7)：523-526,588,5.DOI:10.3969/J.ISSN.1007-6611.2013.07.005
负压对兔前交叉韧带重建术后腱-骨界面血管化影响的研究
Effect of negative pressure on vascularization of tendon-bone interface after reconstruction of anterior cruciate ligament in rabbits
摘要
Abstract
Objective To explore the effect of intermittent negative pressure on vascularization of tendon-bone interface after reconstruction of anterior cruciate ligament in rabbits.Methods One side of hind legs in 24 New Zealand white rabbits were randomly chosen as negative pressure group,and its contralateral hind leg was chosen as control.The anterior cruciate ligament model was reconstructed with autogenous semitendinosus of rabbit.Joint at the negative pressure side was placed with drainage tube connecting the micro-negative pressure aspirator for maintaining a low-intensity,intermittent negative pressure.Control side was placed with ordinary drainage tube.Drainage tubes of both sides were removed at 5 d after operation.After 6 weeks,femur-ligament-tibia complex was obtained to determine tendon graft tension,observe the blood vessels of the tendon-bone interface and detect the expression of VEGF.Results Twenty-three rabbits were included in the study,and one rabbit was excluded because of joint infections.Rally measurement results showed that the maximum load breakage of tendon graft was significantly greater in negative pressure group than that in control group (P ＜ 0.05).Histological studies of tendon-bone interface found that the number of blood vessels was significantly more in negative pressure group than that in control group (P ＜ 0.01).Immunohistochemistry showed that expression of VEGF was higher in negative pressure group than that in control group (P ＜ 0.01).Conclusion Intermittent negative pressure may promote tendon-bone healing by increasing the vascularization of the tendon-bone interface after reconstruction of anterior cruciate ligament in rabbits.关键词
前交叉韧带/负压/腱-骨愈合/血管内皮生长因子Key words
anterior cruciate ligament/ negative pressure/ tendon-bone healing/ vascular endothelial growth factor分类
医药卫生引用本文复制引用
孙正明,凌鸣,冯伟楼,董向辉,刘时璋,易智..负压对兔前交叉韧带重建术后腱-骨界面血管化影响的研究[J].山西医科大学学报,2013,44(7):523-526,588,5.基金项目
陕西省自然科学基础研究计划基金资助项目(2011JM4050) （2011JM4050）
