山西医科大学学报2013，Vol.44Issue(7)：579-581,590,4.DOI:10.3969/J.ISSN.1007-6611.2013.07.020
小鼠灌胃乙酸建立胃溃疡模型的可行性研究
Feasibility of intragastric administration of acetic acid for inducing gastric ulcer model in mouse
摘要
Abstract
Objective To explore the feasibility of intragastric administration of acetic acid for inducing gastric ulcer model in mouse,and explore its reasonable modeling conditions.Methods Sixty mice were randomly divided into six groups according to the concentration of acetic acid:7.34 × 10-4mol/L group,8.74 × 10-5 mol/L group,1.75 × 10-5 mol/L group,8.74 × 10-6 mol/L group and 4.37 × 10-6 mol/L group and saline control group.Each group contained five males and five females.Acetic acid was intragastric administrated at the volume of 0.3 ml each time.After the model was established,the presence and degree of ulcer were observed with naked eyes and microscope.Results Superficial ulcer was found in all mice after 7.34 × 10-4 mol/L acetic acid for one time and 8.74 × 10-5 mol/L for three times.The intragastric administration of 1.75 × 10-5 mol/L acetic acid for 5 times resulted in superficial ulcer in 50％ cells.Under microscope,typical peptic ulcer and the damage of both glands and mucosa were found after 8.74 × 10-6 mol/L for seven times.Only slight edema and congestion in mucosa was found after 4.30 × 10-6 mol/L acetic acid for seven times.Conclusion It's feasible to induce mouse gastric ulcer by intragastric administration of acetic acid.And the degree of gastric ulcer is related to the concentration of acetic acid.关键词
胃溃疡/动物模型/乙酸/灌胃/小鼠Key words
gastric ulcer/ animal models / acetic acid / intragastric administration / mouse分类
医药卫生引用本文复制引用
于嘉兴,杜俊喆,李帅,贾佳,张能,孙艳荣,彭旖旎,张轩萍..小鼠灌胃乙酸建立胃溃疡模型的可行性研究[J].山西医科大学学报,2013,44(7):579-581,590,4.基金项目
山西医科大学大学生创新基金资助项目 （）
