实用医学杂志2013，Vol.29Issue(13)：2081-2083,3.DOI:10.3969/j.issn.1006-5725.2013.13.005
异氟醚对过氧化氢诱导内皮细胞凋亡的保护作用
Protective effect of isoflurane on endothelial cells apoptosis induced by hydrogen peroxide
摘要
Abstract
Objective To investigate the protective effect of isoflurane on the injury of human umbilical vein endothelial cells induced by hydrogen peroxide in vitro and to explore its underlying mechanism.Methods Human umbilical vein endothelial cells were cultured in vitro and were divided into three groups:normal control group (group A),isoflurane group (group B),isoflurane and hydrogen peroxide group (group C) and hydrogen peroxide injury group (group D).Flow cytometry was used to detect cell apoptosis.Western blot analysis was performed to detect HSP27 protein expression.Reverse transcriptase PCR was used to detect HSP27,Bcl-2 and Caspase-3 mRNA expression in the endothelia cells.Results Compared with normal control group,hydrogen peroxide cause endothelial cell apoptosis (P ＜ 0.05).Isoflurane can reduce endothelial cell apoptosis induced by hydrogen peroxide (P ＜ 0.05).HSP27 and Bcl-2 expression increased,while Caspase-3 decreased in isoflurane group.Conclusions Isoflurane can inhibit human umbilical vein endothelial cells apoptosis induced by hydrogen peroxide,the mechanisms may be associated with increased expression of HSP27 protein and Bcl-2 and with decreased expression of caspase-3.关键词
异氟醚/人脐静脉内皮细胞/过氧化氢/氧化损伤/细胞凋亡Key words
Isoflurane/ Human umbilical vein endothelial cells/ Hydrogen peroxide/ Oxidative damage / Apoptosis引用本文复制引用
邹叶青,刘哲,刘川,吴琼,贺文凤..异氟醚对过氧化氢诱导内皮细胞凋亡的保护作用[J].实用医学杂志,2013,29(13):2081-2083,3.基金项目
江西省卫生厅科技计划资助项目(编号:20123066) （编号:20123066）
江西省教育厅科技计划资助项目(编号:GJJ12123) （编号:GJJ12123）
