实用医学杂志2013，Vol.29Issue(13)：2091-2093,3.DOI:10.3969/j.issn.1006-5725.2013.13.009
白藜芦醇对小鼠神经元线粒体mtDNA拷贝数及相关基因表达的影响
Effects of resveratrol on the level of mtDNA and the expressions of mitochondrial corresponding genes in mitochondria in cerebral cortex neurons of mice
摘要
Abstract
Objective To investigate the effects of resveratrol on the biogenesis of mitoehondria in cerebral cortex neurons of mice cultured in vitro.Methods The neurons cultured in vitro were divided randomly into sham group,dimethyl sulfoxide (DMSO) group and resveratrol group.After 7 days of cultivation,the ATP content and mtDNA level in neurons were measured,and the expressions of Tfam,NRF-1,NRF-2 and PGC-1o were evaluated by real time PCR.Results As compared with those in DMSO group,the ATP content and mtDNA level in neurons in resveratrol group were significantly increased (P ＜ 0.01 and P ＜ 0.001).In addition,the mRNA expressions of Tfam,NRF-1,NRF-2 and PGC-lα were enhanced by resveratrol (P ＜ 0.001).Conclusion Resveratrol might potentially promote the biogenesis of mitochondria in cerebral cortex neurons of mice cultured in vitro through affecting the level of mtDNA and the expressions of mitochondrial corresponding genes.关键词
藜芦生物碱类/白藜芦醇/神经元/线粒体/小鼠Key words
Veratrum alkaloids/ Resveratrol/ Neurons/ Mitochondria/ Mice引用本文复制引用
董文鹏,晁晓东,王显悦,毕生辉,于浩,林曦,陆华,高大宽,张卫达..白藜芦醇对小鼠神经元线粒体mtDNA拷贝数及相关基因表达的影响[J].实用医学杂志,2013,29(13):2091-2093,3.基金项目
国家自然科学基金资助项目(编号:30901553) （编号:30901553）
