实用医学杂志2013，Vol.29Issue(14)：2248-2250,3.DOI:10.3969/j.issn.1006-5725.2013.14.004
子宫珠蛋白与猫主要过敏原Fel d1之间关系的生物信息学分析
Bioinformatic analysis of the relationship between uteroglobin and cat major allergen Fel d 1
摘要
Abstract
Objective To explore the relationship between human uteroglobin and cat allergen Fel d 1.Methods Bioinformatic software such as ClustalW 1.83 and MEGA5.1 Beta3 were employed,for sequence alignment and clustering,on the sequences of secretoglobins and the two subunits (P30438 and P30440) of Fel d 1 retrieved from NCBI and SwissProt,supplemented with manual subtraction of accession items.The antigenicity of P30438 and P11684,a typical sequence of uteroglobin were compared by online bioinformatic software NetMHCII 2.2.The 3D structures of P30438,P30440 and P11684 were modeled and deciphered in parallel.Results Secretoglobin family harbored a large amount of redundant sequences.These sequences were first classified into 7 subgroups and were then by progressive clustering reduced to 13 representative non-redundant sequences.P11684 was closely related to the subunit P30438 of Fel d 1.P11684 and P30438 both displayed several high binding affinities with HLA molecules.The 3D structure of P11684 was similar with those of P30438 and P30440.Conclusion Uteroglobin may have the potential allergenicity,as cat allergen Fel d 1 does.关键词
子宫珠蛋白/猫主要过敏原Fel d 1/进化树形图/抗原性/三级结构Key words
Uteroglobin / Cat allergen Fel d 1 / Dendrogram / Antigenicity / Tertiary Structure引用本文复制引用
欧阳穗,黄于艺,凌春芳,何颖,陈惠芳,邹泽红..子宫珠蛋白与猫主要过敏原Fel d1之间关系的生物信息学分析[J].实用医学杂志,2013,29(14):2248-2250,3.基金项目
国家自然基金(编号:30771240) （编号:30771240）
国家科技重大专项重大课题(编号:2011ZX08011-005) （编号:2011ZX08011-005）
呼吸疾病国家重点实验室(广州医学院)课题研究基金 （广州医学院）
