实用医学杂志2013，Vol.29Issue(14)：2265-2267,3.DOI:10.3969/j.issn.1006-5725.2013.14.010
耐受性小鼠骨髓源性树突状细胞的培养及生物学功能
The cultivation of tolerant bone marrow-derived dendritic cells of mice and their biological function in vitro
摘要
Abstract
Objective To explore a relatively simple method of inductive cultivation of tolerant bone marrowderived dendritic cells (DCs) of mice and to investigate the biological function of tolerant DCs.Methods Bone marrow-derived DCs were induced by GM-CSF and IL-4 from the culture of mice bone marrow cells in vitro.The morphology,phenotype,and function of DCs were observed.Results DCs with low expression of surface costimulate molecular in day 7 were in immature state,these DCs could stimulate the proliferation of lymphocytes and the secretion of IL-10.Conclusion The method used in this study was a simple method to harvest tolerant bone marrow-derived DCs,which could stimulate the proliferation of lymphocytes and the secretion of IL-10.关键词
树突状细胞/耐受性/IL-10Key words
Dendritic cells / Tolerance/ IL-10引用本文复制引用
马院院,王春锋,杨木清,丁晶,李济宇..耐受性小鼠骨髓源性树突状细胞的培养及生物学功能[J].实用医学杂志,2013,29(14):2265-2267,3.基金项目
国家自然科学基金(编号:30600598) （编号:30600598）
上海市科学技术委员会医学重点攻关项目(编号:09411952500) （编号:09411952500）
上海市科委青年科技启明星资助计划(编号:10QH1401800) （编号:10QH1401800）
上海市教育委员会曙光计划(编号:10SG20) （编号:10SG20）
国家自然科学基金(编号:81270555) （编号:81270555）
