实用医学杂志2013，Vol.29Issue(14)：2383-2385,3.DOI:10.3969/j.issn.1006-5725.2013.14.057
LAMP结合基因芯片技术在快速检测嗜肺军团菌中的应用
A rapid detection of Legionella Pneumophila by LAMP in combination with gene chip
杨俊发 1许飞2
作者信息
- 1. 330006 南昌大学医学院研究生部
- 2. 330006 南昌大学第一附属医院呼吸内科
- 折叠
摘要
Abstract
Objective To establish a rapid and accurate method to detect the Legionella Pneumophila (LP).Methods The loop-mediated isothermal amplification(LAMP) to amplify the specific DNA fragments of LP and the gene chip technology was further used to identify the LP sequence.Results The DNA bands corresponding to LP DNA could be specifically amplified by the LAMP reaction.For normal PCR,the minimum dilution of genomic DNA was 10-5,however,for the LAMP,the minimum dilution of genomic DNA was up to 10-7,so the sensitivity of the LAMP reaction is about 100 times higher than of normal PCR.The combination of the LAMP and gene chip contributed to demonstrating the specific LP sequence signal.Conclusion Combination of LAMP and gene chip is a simple,accutate and rapid technology to detect LP with,high sensitivity and specificity.关键词
环介导等温核酸扩增技术/基因芯片/嗜肺军团菌/检测Key words
LAMP/ Gene chip / Legionella Pneumophila / Detection引用本文复制引用
杨俊发,许飞..LAMP结合基因芯片技术在快速检测嗜肺军团菌中的应用[J].实用医学杂志,2013,29(14):2383-2385,3.
