生殖医学杂志2013，Vol.22Issue(7)：517-522,6.DOI:10.3969/j.issn.1004-3845.2013.07.011
顺式高尔基体蛋白GM130影响小鼠卵母细胞纺锤体组装
GM130, a cis-Golgi protein, regulates spindle assembly during mouse oocyte meiotic maturation
摘要
Abstract
Objective:To identify the role of GM130,a cis-Golgi protein,in regulating meiotic spindle assembly.Methods:GM130 localization was determined by immunofluorescence and confocal microscopy.The expression of GM130 was detected by immunoblotting analysis.Results:The results showed that GM130 was localized to the spindle poles at both metaphase Ⅰ and metaphase Ⅱ stages and associated with the midbody at telophase Ⅰ stage.It showed that GM130 was expressed at all stages by immunoblotting.By nocodazole treatment,it clarified that GM130 localization was consistently dependent on spindle assembly.It also showed that knockdown of GM130 caused abnormal spindle formation and impaired the localization of microtubule organizing center (MTOCs) proteins γ-tubulin and Plk1.Conclusions:GM130 may act as a centrosome-associated protein to regulate spindle assembly.关键词
GM130/小鼠卵母细胞/减数分裂/纺锤体Key words
GM130/ Mouse oocyte/ Meiotic/ Spindle引用本文复制引用
张春晖,钱卫平,孙洪梅,孟繁华..顺式高尔基体蛋白GM130影响小鼠卵母细胞纺锤体组装[J].生殖医学杂志,2013,22(7):517-522,6.基金项目
国家自然科学基金(2012-81200438) （2012-81200438）
