生殖医学杂志2013，Vol.22Issue(8)：596-601,6.DOI:10.3969/j.issn.1004-3845.2013.08.009
顺式高尔基体蛋白GM130影响小鼠卵母细胞减数不对称分裂的研究
A cis-Golgi protein GM130 regulates asymmetric division in mouse oocyte
摘要
Abstract
Objective:To identify the role of GM130 in regulating MI spindle migration and the meiotic asymmetric division.Methods:GM130 localizations were determined by immunofluorescence and confocal microscopy.Microtubule dynamics were filmed on Perkin Elmer precisely Ultra VIEW VOX confocal Imaging System.Results:A large proportion of oocytes extruded an abnormally large polar body (PB) in the GM130-MO injected group,and the enlarged PB contained a spindle with normal morphology in some cases.In many cases,the oocyes arrested at MI stage.It was observed by using live cell imaging that depletion of GM130 affected spindle migration and resulted in elongated spindle and large polar body extrusion.It was further found that depletion of GM130 blocked p-MEK1/2 accumulation at the spindle poles.And the results showed that GM130 detached from the spindle poles in oocytes treated with MEK specific inhibitor U0126.Conclusions:GM130 may cooperate with the MAPK pathway to play roles in spindle migration and asymmetric division during mouse oocyte maturation.关键词
顺式高尔基体蛋白GM130/小鼠卵母细胞/减数不对称分裂/纺锤体Key words
GM130/ Mouse oocyte/ Asymmetric division/ Spindle migration引用本文复制引用
张春晖,钱卫平,孙洪梅,孟繁华..顺式高尔基体蛋白GM130影响小鼠卵母细胞减数不对称分裂的研究[J].生殖医学杂志,2013,22(8):596-601,6.基金项目
国家自然科学基金(2012-81200438) （2012-81200438）
