生殖医学杂志2013，Vol.22Issue(8)：602-607,6.DOI:10.3969/j.issn.1004-3845.2013.08.010
卵巢早衰小鼠中抗苗勒管激素与卵泡刺激素受体相关性的研究
Relationship between anti-Müllerian hormone level and expression of follicle-stimulating hormone receptor in premature ovarian failure mice
摘要
Abstract
Objective:To study the relationship between anti-Müllerian hormone (AMH) level and expression of follicle-stimulating hormone receptor (FSHR) in mice with premature ovarian failure.Methods:The peptide consisting of the first 330-342 amino acid sequences of mouse zona pellucida 3 was synthesized and used as immunogen to immunize Balb/c female mice of specific pathogen free (SPF) grade with subcutaneous injection to establish the model of premature ovarian failure (POF).FSH and E2 levels in serum were measured by radioimmunoassay.The AMH and anti-zona pellucida antibody (AzpAb) levels in the serum were measured by enzyme-linked immunosorbent assay (ELISA).The ovarian tissue was stained with HE.The FSHR expression in the ovarian granulosa cells was analyzed by immunohistochemistry.Results:Increased FSH and AzpAb levels,decreased E2 and AMH levels and follicular atresia were found in POF mice.A few normal development follicles were observed in POF mice.The expression of FSHR was only seen in the granulosa cells of follicle and the FSHR expression was less in POF mice.Conclusions:In POF model mice,the expression of FSHR is only in the granulosa cells of follicle,and its expression being lower.The AMH concentration also is lower,and in the AMH low concentration range,along with AMH concentration decreasing,FSHR expression was also reduced in granulosa cells.关键词
卵巢早衰/抗苗勒管激素/卵泡刺激素受体Key words
Premature ovarian failure/ Anti-Müllerian hormone/ Follicle-stimulating hormone receptor引用本文复制引用
张迪,桑丽英,赵花,崔蓉,苗竹林,钟兴明,韦相才..卵巢早衰小鼠中抗苗勒管激素与卵泡刺激素受体相关性的研究[J].生殖医学杂志,2013,22(8):602-607,6.基金项目
广东省科技计划项目(2009B030801270) （2009B030801270）
广东省自然科学基金项目(10151008010000000) （10151008010000000）
