生殖医学杂志2013，Vol.22Issue(8)：608-611,4.DOI:10.3969/j.issn.1004-3845.2013.08.011
冻融成人卵巢组织异种移植后形态和功能的研究
A study on the morphology and function of adult ovarian tissues after cryopreservation and xenotransplantation
摘要
Abstract
Objective:To explore the restoration of ovarian estradiol (E2) secretion and the development of follicles after subcutaneous xenotransplantation of human ovarian tissue cryopreserved by vitrification.Methods:Thirty SCID mice were divided into 2 groups:ovariectomized control group and xenografted ovarian group.Seven days later,each mouse was injected with 5 U of rFSH and 5 U of rLH every two days for 20 weeks.On 20 weeks after operation,the grafted ovarian tissues were obtained and estimated under microscopy.The weight of uterus and the level of E2 were measured.Results:The proportions of growing follicles were found in the survival grafts.The weight of uterus and the E2 levels in the xenografted ovarian group were significantly higher than those in the ovariectomized control group (P＜0.05).Conclusions:The vitrification frozen-thawed adult ovarian tissues can survive in mouse.The follicles could develop and E2 well secrete in xenografted ovarian transplanted into the mouse subcutaneous tissue.关键词
卵巢组织/玻璃化/移植Key words
Ovarian tissue/ Vitrification/ Transplantation引用本文复制引用
牛晓明,史蓬,霍世伟..冻融成人卵巢组织异种移植后形态和功能的研究[J].生殖医学杂志,2013,22(8):608-611,4.
