畜牧兽医学报2013，Vol.44Issue(7)：1000-1007,8.DOI:10.11843/j.issn.0366-6964.2013.07.002
猪BPI基因部分cSNPs检测及其对蛋白结构功能的影响
The Partial cSNPs Detection of Pig BPI Gene and Its Influence on Protein Structure and Function
摘要
Abstract
This study was conducted to detect the single nucleotide polymorphism (SNPs) of BPI gene in partial coding region in ninety-eight Sutai pigs,and to predict the effect of mutation on BPI protein structure and function.PCR-SSCP/RFLP combined with sequencing were used to detect the SNPs in exon 1,exon 4 and exon 10 of BPI gene,and bioinformatics approach was used to predict the physicochemical properties and structures of original protein or mutant protein.Refer to the cDNA sequence of BPI gene,six SNPs existed in exon 1,exon 4 and exon 10 of BPI gene,with three synonymous mutations(c.24A＞G,c.54T＞C and c.522C＞T) and three missense mutations (c.433C ＞ T (Arg145Trp),c.1060A ＞ G (Thr354Ala) and c.1151T ＞ G (Leu384Arg)).Three missense mutations had no influence on the physicochemical properties,signal peptide,transmembrane region,glycosylation sites,secondary structure and tertiary structure of the BPI protein,but led to the change of its phosphorylation sites or the existence of other potential function sites domain.The result of Western blot experiment indicated that there was an target band at 53 ku.It was obvious that expression of BPI protein in ETEC F18-resistant group was significant higher than that in susceptible group.The base mutations in exon 4 and exon 10 of pig BPI gene affect protein function in one way or another,which may have an influence on resistance/susceptibility of the pig disease.关键词
猪/BPI基因/SNPs/生物信息学/蛋白结构和功能Key words
pig/ BPI gene/ SNPs/ bioinformatics/ protein structure and function分类
农业科技引用本文复制引用
吴正常,王靖,朱世平,赵乔辉,刘璐,訾臣,吴圣龙,包文斌..猪BPI基因部分cSNPs检测及其对蛋白结构功能的影响[J].畜牧兽医学报,2013,44(7):1000-1007,8.基金项目
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
国家自然科学基金(31172183) （31172183）
江苏省产学研前瞻性联合研究项目(BY2012157) （BY2012157）
江苏省科技支撑计划(农业)(BE2010371 （农业）
BE2012330 （）
苏州市科技支撑计划(SN201013 （）
SN201133)) （）
