畜牧兽医学报2013，Vol.44Issue(7)：1057-1062,6.DOI:10.11843/j.issn.0366-6964.2013.07.010
羊驼皮肤中可溶性鸟苷酸环化酶的表达差异
The Different Expression of Soluble Guanylate Cyclase in Alpaca Skin
摘要
Abstract
To investigate the function of soluble guanylate cyclase (sGC) in alpaca skin and coat color regulation,differential expression of sGC in skin of brown versus white alpacas was examined using quantitative real-time PCR(qRT-PCR),Western blotting and immunohistochemistry.Expression of sGC mRNA was lower (0.16 fold) in skin samples from brown alpacas relative to expression in skin of white alpacas with significant difference (P＜0.01).Using a rabbit polyclonal sGC antibody,an immune reactive band corresponding to sGC was detected by Western blotting analysis in skin samples from white and brown alpacas,showing a weaker signal detected in the samples from brown alpacas with significant difference (P＜0.01).The results of immunohistochemistry revealed that sGC was localized in the cytoplasm and intercellular substance of upper hair papilla in hair follicles of alpacas with white hair color,but was localized in ytoplasm and intercellular substance of lower hair bulb and outer root sheath cells in hair follicles of alpacas with brown hair color.Observed differential expression and localization of sGC in skin of white versus brown alpacas suggests a potential role in hair color regulation.关键词
可溶性鸟苷酸环化酶/皮肤/cAMP/羊驼Key words
soluble guanylate cyclase (sGC) / skin/ cAMP/ alpaca分类
农业科技引用本文复制引用
张俊珍,范瑞文,杨姗姗,石占全,姬凯元,董常生..羊驼皮肤中可溶性鸟苷酸环化酶的表达差异[J].畜牧兽医学报,2013,44(7):1057-1062,6.基金项目
山西省科技攻关项目(20120311034-2) （20120311034-2）
国家自然科学基金(30671512) （30671512）
