畜牧兽医学报2013，Vol.44Issue(7)：1117-1123,7.DOI:10.11843/j.issn.0366-6964.2013.07.017
PRRS聚乳酸乙醇酸微粒DNA疫苗的制备及其免疫原性
Preparation and Immunogenicity of Ploy (Lactide-Co-Glycolide) Microparticles DNA Vaccine against Porcine Reproductive and Respiratory Syndrome
摘要
Abstract
To enhance immune response induced by DNA vaccines,porcine reproductive and respiratory syndrome (PRRS) DNA vaccines pCI-ORF5 was adsorbed on the surface of poly(lactide-co-glycolide) (PLGA) microparticles.The microparticles were prepared using a solvent evaporation method and the adsorption amount,in vitro release profiles and in vivo immunogenicity were evaluated.The results indicated that the DNA adsorbed on PLGA microparticles was about 0.9％ in 6 hours,and the release behaviors were influenced by some parameters such as CTAB content,PLGA molecular weight,PLGA concentration,internal aqueous phase volume.The PLGA microparticles with adsorbed DNA induced significantly enhanced humoral and cellular response in comparison to naked DNA in mice.It suggests that PLGA microparticles could be a promising vector for the delivery of DNA vaccines.关键词
猪繁殖与呼吸综合征/DNA疫苗/聚乳酸乙醇酸/DNA递送Key words
porcine reproductive and respiratory syndrome/ DNA vaccine/ poly(lactide-co-glycolide) / DNA delivery分类
农业科技引用本文复制引用
张辉,王光利,韩秀英,周磊..PRRS聚乳酸乙醇酸微粒DNA疫苗的制备及其免疫原性[J].畜牧兽医学报,2013,44(7):1117-1123,7.基金项目
安徽省自然科学研究基金(KJ2010B192 （）
KJ2011B149) （）
安徽省优秀青年人才基金(2010SQRL082) （2010SQRL082）
