畜牧兽医学报2013，Vol.44Issue(7)：1124-1130,7.DOI:10.11843/j.issn.0366-6964.2013.07.018
抗菌肽BuforinⅡ衍生物对金黄色葡萄球菌细胞膜作用机制的研究
The Action Mechanism of Two Analogues of the Antimicrobial Peptide Buforin Ⅱ on Staphylococcus aureus Membrane
摘要
Abstract
In this paper,the action mechanism of BF2-A/B,two analogues of antimicrobial peptide Buforin Ⅱ,on Staphylococcus aureus membrane had been researched.The results of FACS-can analysis implied that BF2-A/B did not induce the influx of PI into the S.aureus cells.The rate of positive cells stained by fluorescent probe after BF2-B treatment was slightly higher than that of BF2-A.These electron micrographs showed that after 20 min of treatment by BF2-A,S.aureus cells still retained the plasma membrane integrality; however,BF2-B could cause some slight leakages of cellular cytoplasmic contents.The results of FACS analysis displayed that both the peptides could penetrate the cells,and BF2-B penetrated the cells more efficiently.The visualization of cofoocal microscopy proved that FITC-labeled BF2-A and BF2-B penetrated the bacterial cell membrane and accumulated in the cytoplasm of the cell immediately.The results of research demonstrated that BF2-A/B didn't destroy the cell membrane of G+ bacteria,and then exert the antimicrobial activity after influx into cytoplasm.BF2-B slightly disturbed cell membrane causing influx of PI and leakage of cytoplasmic contents during peptide crossing phospholipids bilayer.Meanwhile,the cell-penetrating efficiency of BF2-B was accordingly enhanced,which caused that BF2-B displayed more excellent antimicrobial activity to S.aureus.关键词
抗菌肽/BuforinⅡ/金黄色葡萄球菌/细胞膜/作用机制Key words
antimicrobial peptides/ Buforin Ⅱ / Staphylococcus aureus / membrane/ mechanism of action分类
农业科技引用本文复制引用
郝刚,乐国伟,施用晖..抗菌肽BuforinⅡ衍生物对金黄色葡萄球菌细胞膜作用机制的研究[J].畜牧兽医学报,2013,44(7):1124-1130,7.基金项目
国家自然科学基金(30871805 （）
31172214) （）
中央高校基本科研业务基金(11NZYQN30) （11NZYQN30）
十二五支撑计划(2012BAD33B05) （2012BAD33B05）
