畜牧兽医学报2013，Vol.44Issue(7)：1160-1166,7.DOI:10.11843/j.issn.0366-6964.2013.07.023
输卵管上皮细胞共培养对小鼠早期胚胎发育阻滞的影响
Effect of Oviduct Epithelial Cells Co-culture on the Developmental Arrest of Early Embryos in Mouse
摘要
Abstract
This experiment was conducted to study the effect of oviduct epithelial cells co-culture on the development of early embryos,and explored the mechanisms of oviduct epithelial cells coculture on overcoming the developmental arrest in mouse.Mouse oviduct epithelial cell line was purified and used to co-culture the zygotes,H2O2 contents in embryos were measured by DCHF-DA,MTT was used to measure the activity of epithelial cells,RT-PCR was applied to test the transcriptions of CAT and GPX genes.Oviduct epithelial cells co-culture can significantly improve the early 2-cell and 4-cell rates (P＜0.05),the blastocyst rate was also increased (P＞ 0.05).Oviduct epithelial cells co-culture can decrease the H2O2 content in early 2-cell embryos (P＜0.05).Also there was a deposit of H2O2 in the arrested embryos,the rates of type Ⅰ and Ⅱ arrested embryos were decreased(P＞0.05),type Ⅲ was increased(P＜0.05) by oviduct epithelial cells co-culture.On the other side,the cellular activity was improved (P＞0.05) when embryos developed to early 2-cell phase,at the same time,transcriptions of CAT and GPX were increased (P＞0.05).Those results indicated that oviduct epithelial cell co-culture can improve the development of embryos by cell-cell contact to decrease the reactive oxygen species content in embryos,overcome developmental arrest and promote embryo development.关键词
输卵管上皮细胞/共培养/早期胚胎/阻滞/活性氧Key words
oviduct epithelial cells/ co-culture/ early embryos/ arrest/ ROS分类
农业科技引用本文复制引用
庄利利,吴俊波,胡德宝,蔡丽娟,张也,李钟淑,方南洙..输卵管上皮细胞共培养对小鼠早期胚胎发育阻滞的影响[J].畜牧兽医学报,2013,44(7):1160-1166,7.基金项目
延边大学211工程第三期重点学科建设项目(2009-002) （2009-002）
动物高效快繁及胚胎工程高新技术研究 （）
