畜牧兽医学报2013,Vol.44Issue(7):1167-1172,6.DOI:10.11843/j.issn.0366-6964.2013.07.024
青藏高原部分地区牦牛源沙门菌血清型及毒力基因的调查
The Survey Study on the Serovar and Virulent Gene of Yak Salmonella from Some Areas of Qinghai-Tibetan Plateau
摘要
Abstract
The aim of this study was to investigate the distribution of serovars and virulent genes of yak Salmonella from the some areas of the Qinghai-Tibetan pleteau.The serotyping result exhibited that the 31 yak Salmonella isolates were divided into 8 different serovars,S.Dublin (32.26%),S.Enterica (29.03%) and S.Typhimurium (12.90%) were dominant serovars,other are S.Blegdam,S.Onarimon,S.Stanely,S.Orion and S.Newport.In addition,the 14 different virulent genes were detected in 31 yak Salmonella isolates by PCR method.The results showed all isolates carried virulent genes of the pathogenicity island-1 (SPI-1) to SPI-5,and the five serovars yak Salmonella isolates carried the virulence plasmid.It is noteworthy that the S.Blegdam and S.Onarimon were first found to possess the virulence plasmid.Therefore,the high carrying rate of virulence plasmid was occurred in yak Salmonella suggested that the yak Salmonella might have strong pathogenicity,which will provide important information to further study the pathogenicity of yak Salmonella from the Qinghai-Tibetan pleteau关键词
牦牛/沙门菌/血清型/毒力基因Key words
yak/ Salmonella/ serovar/ virulent gene分类
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张斌,朱晓霞,岳华,汤承..青藏高原部分地区牦牛源沙门菌血清型及毒力基因的调查[J].畜牧兽医学报,2013,44(7):1167-1172,6.基金项目
川西北牧区"生产生态生活"优化保障关技术集成与示范(2012BAD13B06) (2012BAD13B06)