新医学2013，Vol.44Issue(6)：430-433,4.DOI:10.3969/g.issn.0253-9802.2013.06.018
二氯乙酸对急性髓系白血病细胞株U937细胞凋亡的影响及其机制的研究
Effect of dichloroacetate on apoptosis of human leukemia cell line U937
刘伟才1
作者信息
- 1. 528100佛山,广东佛山市三水区人民医院检验科
- 折叠
摘要
Abstract
Objective To study the effect of DCA on apoptosis of human leukemia cell line U937 and possible mechanism.Methods The leukemia cell line U937 cells were cultured with various concentration of dichloroacetate in different time duration.The proliferation ability was measured by cck8 assay.The reactive oxygen species (ROS) and apoptosis rate were detected by flow cytometry.The expression of cleavedcaspase 3 and cleaved-PARP were shown by western blot.Results The proliferation of U937 cells could be inhibited by DCA.The apoptosis of U937 cells were increased and could be caused by up-regulated ROS when treated with DCA for 24 h.Conclusions The proliferation of U937 cells could be inhibited by DCA.Mitochondrial pathway may be involved in the apoptosis of U937 cells by DCA.ROS may played an important role in apoptosis of U937 cells.关键词
二氯乙酸/U937/细胞凋亡/肿瘤代谢/活性氧家族Key words
Dichloroacetate/ U937 / Apoptosis/ Cancer Metabolism/ Reactive oxygen species引用本文复制引用
刘伟才..二氯乙酸对急性髓系白血病细胞株U937细胞凋亡的影响及其机制的研究[J].新医学,2013,44(6):430-433,4.
