新医学2013，Vol.44Issue(7)：463-466,4.DOI:10.3969/g.issn.0253-9802.2013.07.007
广东省艾滋病长期抗病毒治疗患者耐药情况调查
Investigation of HIV-1 drug resistance prevalence in AIDS patients with long-term HAART therapy in Guangdong Province
于国龙 1鄢心革 1秦冰 2李杰 1刁丽梅 1况轶群 3付笑冰 1颜瑾 1林鹏1
作者信息
- 1. 511430广州,广东省疾病预防控制中心艾滋病防治研究所
- 2. 510440广州,广东省生物制品与药品研究所
- 3. 511430广州,广东省公共卫生研究院
- 折叠
摘要
Abstract
Objective To find the HIV-1 drug resistance prevalence in AIDS patients with HAART therapy over four years.Methods Recruited the HIV/AIDS patients with HAART for more than four years,counted CD4 + T lymphocyte and HIV-1 viral load; and then detected the genotype drug resistance when the samples with virus load more than 1000 copies/ml by the IN-house method.Firstly amplified the whole genome of HIV-1 protease and part of reverse transcriptase genome,secondly sequenced the PCR products,and then assembled the sequence.Blast the sequence with the Stanford HIV-1 Drug Resistance Database and get the drug resistance mutation and drug resistance.Results CD4 + T Lymphocytes partly reconverted again and the virus load uncontrolled is 18.3％; about 10％ patients (12 patients) were with high genotypic resistance.Major drug resistance mutations in RT region are as follows:Y181C、G190A、K101Q/E/N、M184IMV、K219KR.No major mutation was found in PI region.The NNTRI drug resistance is 100％,92％ to NVP and EFV respectively.The NTRI Drug resistance to 3TC、FTC and ABC is 83％,83％ and 42％ respectively.Conclusions HIV-1 drug resistance in therapy patients for long time was already more than 10 percent in Guangdong province.Viruses with high drug resistance to NNTRI and NTRI were found in this population,and more measure should be done in these people.关键词
艾滋病/高效抗逆转录病毒治疗/人类免疫缺陷病毒/耐药Key words
Acquired immunodeficiency syndrome/ Highly active antiretroviral therapy/ Human immunodeficiency virus infection/ Drug resistance引用本文复制引用
于国龙,鄢心革,秦冰,李杰,刁丽梅,况轶群,付笑冰,颜瑾,林鹏..广东省艾滋病长期抗病毒治疗患者耐药情况调查[J].新医学,2013,44(7):463-466,4.
