应用化学2013，Vol.30Issue(8)：922-926,5.DOI:10.3724/SP.J.1095.2013.20485
京尼平交联磁性壳聚糖微球的制备及其脂肪酶的固定化
Preparation of Genipin Cross-linked Magnetic Chitosan Microsphere and Its Immobilization on Lipase
摘要
Abstract
Inverse suspension combined with the sol-gel method was deployed to prepare a magnetic chitosan microspheres.The lipase was immobilized on magnetic chitosan microspheres with genipin as the cross-linking reagent.The optimum conditions and properties of immobilization enzyme were investigated.The maximal specific activity of 4.31 U/g was achieved when the crosslinking reaction was carried out at 55 ℃ for 8 h using 0.6 g/L genipin.The optimum pH,temperature and Km of the immobilized enzyme were 8,25 ～ 35 ℃ and 0.26 mol/L,respectively.The immobilized lipase showed improved thermal stability and remained active in a broader pH range,compared with those of free lipase.Such immobilized lipase can be recycled via the magnetic separation for a certain extent.关键词
磁性壳聚糖微球/京尼平/固定化酶Key words
magnetic chitosan microspheres/ genipin/ immobilized enzyme分类
化学化工引用本文复制引用
唐荣华,段玮,陈波..京尼平交联磁性壳聚糖微球的制备及其脂肪酶的固定化[J].应用化学,2013,30(8):922-926,5.基金项目
国家"863"课题(2010AA023001)、国家自然科学基金(20927005,21275049,81274178)、湖南省科技计划课题(2010TT1001)和湖南省高校科技创新团队支持计划资助 （2010AA023001）
