中国骨伤2013，Vol.26Issue(7)：597-600,4.DOI:10.3969/j.issn.1003-0034.2013.07.016
慢病毒介导SOX9基因转染骨髓间充质细胞中的基因表达
Gene expression of bone mesenchymal stem cells transduced by the lentiviral vector of SOX9 gene
摘要
Abstract
Objective:To construct one lentiviral vector containing mouse SRY-related high mobility group-box gene 9 (SOX9) and transfect the murine bone mesenchymal stem cells (mBMSCs) in vitro and observe the expression of target gene.Methods:RNA from the vectors containing mouse SOX9 gene were extracted and SOX9 genes were amplified by reverse transcription-Polymerase Chain Reaction (RT-PCR).The SOX9 genes were connected into lentiviral vectors pGC-FU.Then pGC-FU-SOX9 transduced into 293T cells to produce recombinant lentivirus called as Lenti-SOX9-EGFP.mBMSCs were transfected.The expression of target gene was detected by immunofluorescence,RT-PCR and Western Blot.Results:LentiSOX9-EGFP was recombined successfully and transduced efficiently into mBMSCs.The expression of SOX9 gene was confirmed by RT-PCR and Western Blot.Conclusion:Lentiviral vector of mouse SOX9 gene can transfect successfully into mBM-SCs.Meanwhile,SOX9 gene may be expressed in mBMSCs.This will provide the target cells for the following study about SOX9 gene repairing cartilage injury.关键词
骨关节炎/细胞介导免疫/慢病毒属/SOX9基因/骨髓间充质细胞Key words
Osteoarthritis / Cell-mediated immunity / Lentivirus / SOX9 gene / Bone mesenchymal stem cells引用本文复制引用
白洁玉,梁大川,程鹏,杨自权,卫小春..慢病毒介导SOX9基因转染骨髓间充质细胞中的基因表达[J].中国骨伤,2013,26(7):597-600,4.基金项目
国际科技合作项目(编号:2010DFA32450) （编号:2010DFA32450）
国家自然科学基金资助项目(编号:30973048) （编号:30973048）
国家人事部及山西省人事厅留学回国人员科技活动择优资助项目 （）
山西省留学基金项目(编号:107) （编号:107）
山西省自然科学基金资助项目(编号:2010011050-6) （编号:2010011050-6）
International Scientific and Technological Cooperation Program (No.2010DFA32450) （No.2010DFA32450）
