中国临床药学杂志2013，Vol.22Issue(4)：230-233,4.
RP-HPLC法测定人血浆中氯沙坦钾、厄贝沙坦和格列齐特浓度
Simultaneous determination of losartan, irbesartan and gliclazide in human plasma by RP-HPLC
摘要
Abstract
AIM To establish a RP-HPLC quantitative analysis method for the determination of losartan,irbesartan and gliclazide in human plasma.METHODS The sample was treated by adding phenytoin sodium as an internal standard.The plasma was extracted with ethyl acetate in acidic condition.The solvent was evaporated to dryness by N2.The residue was dissolved and analyzed by RP-HPLC.The RP-HPLC was conducted on a Hypersil ODS-C18 column (200 mm× 4.6 mm,5μm).The mobile phase was acetonitrile and 0.02 mol· L-1 NaH2PO4 buffer solution (adjusted pH to 2.98 with phosphoric acid) in the gradient elution.The flow rate was 1.0 mL· min-1,and the detection wavelength was 228 nm.RESULTS Using this method,the retention time of losartan,irbesartan,gliclazide,and phenytoin sodium was about 8.797,11.350,14.084 and 5.525 min,respectively.The linear ranges of losartan were 0.05-0.6 mg· L-1 (r =0.999 7),the limit of detection was 0.01 mg· L-1.The linear ranges of irbesartan were 0.1-8.0 mg· L-1 (r =0.999 9),the limit of detection was 0.03 mg·L-1.The linear ranges of gliclazide were 0.1-12.0 mg·L-1(r =0.999 9),the limit of detection was 0.03 mg·L-1.The extraction recovery rate was more than 80％,and the relative standard deviation of intra-day and inter-day was less than 10％,respectively.CONCLUSION The proposed method is simple,accurate and specific for the determination of losartan,irbesartan and gliclazide.关键词
氯沙坦钾/厄贝沙坦/格列齐特/血药浓度/反相高效液相色谱法Key words
losartan/ irbesartan/ gliclazide/ plasma drug concentration/ RP-HPLC引用本文复制引用
田自有,陈赛贞,徐利君,蒋玲燕,张斌,郑斌斌..RP-HPLC法测定人血浆中氯沙坦钾、厄贝沙坦和格列齐特浓度[J].中国临床药学杂志,2013,22(4):230-233,4.基金项目
浙江省药学会、台州市科技局项目(编号2010zyyzz、102ky10-5) （编号2010zyyzz、102ky10-5）
