中国脑血管病杂志2013，Vol.10Issue(7)：379-382,4.DOI:10.3969/j.issn.1672-5921.2013.07.010
米力农对兔蛛网膜下腔出血后脑血管痉挛的作用及其机制研究
Effect of milrinone on cerebral vasospasm after subarachnoid hemorrhage in rabbits and its pathogenesis study
摘要
Abstract
Objective To investigate the effect of milrinone on cerebral vasospasm after subarachnoid hemorrhage (SAH) in rabbits and its pathogenesis.Methods A total of 32 3-month-old Japanese white rabbits were selected and randomly allocated into SAH,control,milrinone,and milrinone + N-nitroL-arginine methyl ester (L-NAME) groups (n =8 in each group).A SAH model was induced by double injection of autologous blood into the cisterna magna.Iisotonic saline was injected into the cisterna magna instead of autoblood in the control group.The others were the same as the SAH model group.At 30 minutes after SAH modeling,milrinone solution 5 mL (30 μg/kg) were injected via ear vein in the milrinone group ;and milrinone solution 5 mL (30 μg/kg) were injected first,then L-NAME 5 mL (30 μg/kg) were injected in the milrinone + L-NAME group; 5 mL isotonic saline were injected in the control and SAH groups.The 4 groups were administered continuously for 3 days,once a day.CT angiography (CTA) and transcranial Doppler (TCD) were used to observe basilar artery diameter and blood flow velocity of the animals in each group at day 3 after procedure.Results TCD examination showed that the mean flow velocities of the basilar arteries in the control,SAH,milrinone,milrinone + L-NAME groups were 19.4 ±-1.8,32.7 ±4.2,17.8 ± 1.8,and 33.2 ±7.2 cm/s,respectively; the peak systolic velocities were 27 ± 5,45 ±4,29 ± 3,and 44 ± 4 cm/s,respectively.CTA examination showed that the diameters of basilar arteries were 1127 ± 140,772 ± 116,1113 ± 101,and 802 ± 91 μm,respectively.In comparision with the above indicators among each group,the differences were statistically significant (P ＜ 0.01).Conclusion Milrinone has a mitigative effect on cerebral vasospasm after SAH,and its vasodilator effect may be associated with L-NAME inhibit the activities of carbon monoxide synthase.关键词
蛛网膜下腔出血/脑血管痉挛/一氧化氮合酶/兔/米力农Key words
Subarachnoid hemorrhage/Cerebral vasospasm/Nifric oxide symthase/Rabbits/Milrinone引用本文复制引用
王立君,邵正凯,牟青春,梁绍栋,王闯,李金库..米力农对兔蛛网膜下腔出血后脑血管痉挛的作用及其机制研究[J].中国脑血管病杂志,2013,10(7):379-382,4.基金项目
黑龙江省卫生厅科研课题(2011-294) （2011-294）
