中国普通外科杂志2013，Vol.22Issue(6)：742-746,5.DOI:10.7659/j.issn.1005-6947.2013.06.016
抑制survivin表达对肝癌细胞阿霉素化疗敏感性的影响
Influence of survivin expression on chemotherapy sensitivity of liver cancer cells to doxorubicin
摘要
Abstract
Objective:To investigate the influence of survivin gene silencing on chemotherapy sensitivity of drug resistant cell line of human hepatocellular carcinoma to doxorubicin.Methods:Three siRNA sequences targeting human survivin gene were designed and synthesized,and then were transfected into hepatocellular carcinoma BEL-7402 cells (3 groups of cells) by liposome-mediated method,respectively.Using the untransfected BEL-7402 cells as control,the expressions of survivin gene and protein as well as sensitivity to doxorubicin in each group of cells were determined by RT-PCR method,immunocytochemical staining and MTT assay,respectively.Results:Compared with control group of cells,both survivin gene and protein expressions in each transfected group of cells were significantly decreased (all P＜0.05).The value of half inhibitory concentration (ICs0) of doxorubicin for control group of cells was (20.60±2.86) μg/mL,while the IC50 values for the three groups of transfected cells were (11.53± 1.46) μg/mL,(14.13± 1.82) μg/mL and (15.53±0.46) μg/mL respectively,all of which had statistical differences versus control (all P＜0.05).Conclusion:Inhibition of survivin gene expression can increase the chemotherapy sensitivity of BEL-7402 cells to doxorubicin.关键词
癌,肝细胞/RNA干扰/抗药性,肿瘤/存活素Key words
Carcinoma, Hepatocellular/ RNA Interference/ Drug Resistance, Neoplasm/ Survivin分类
医药卫生引用本文复制引用
杨永光,刘丽娟,李明意,林满洲,张谷裕..抑制survivin表达对肝癌细胞阿霉素化疗敏感性的影响[J].中国普通外科杂志,2013,22(6):742-746,5.基金项目
广东省卫生厅医学科研基金资助项目(B2008145) （B2008145）
广东医学院科研基金资助项目(2008) （2008）
湛江市科技攻关资助项目(2012C3102025). （2012C3102025）
