中国输血杂志2013，Vol.26Issue(6)：525-529,5.
滤除白细胞对全血保存质量的影响
Effects of leukodepletion filtration on the quality of whole blood
摘要
Abstract
Objective To explore the effects of leukodepletion filtration on the quality of whole blood.Methods Each of 5 units of fresh whole blood (400 mL) were equally divided into 2 aliquots.One subunit was used as filtered group which was filtered by leukocyte deplete filter within 6 hours after blood collection,while the other unit of the pair was used as the control without filtration.Each subunit of 2 groups was separated into 6 parts (30 ～ 40 mL each) in sterile condition and stored at (4 ±2) ℃ for 35 days.Blood routine,pH,free hemoglobin(FHb),P50,adenosine triphosphate(ATP),2,3-diphos-phate glyceric acid (2,3-DPG),electrolytes,the expression of PS and CD47 on red cell membrane,glucose and lactic acid of blood were evaluated on the 1st,7th,14th,21st,28th and 35th day,respectively.Then the storage qualities between the 2groups were compared by statistics.Results The number of residual white blood cells after filtration was (0.32 ± 0.05) ×106.The values of HGB (g/L),MCV (fL),pH,P50 (mmHg),ATP (μmol/gHb),2,3-DPG (mmol/L),the expression of PS (％) and CD47 (％) of filtered group and control group on the 35th day were as follows:(149.00 ± 11.53) vs (137.20 ±3.96),(98.90±3.23) vs(98.96 ±2.10),(6.78 ± 0.02)vs(6.75 ±0.02),(15.36 ± 0.63) vs(15.18 ±0.22),(3.57 ±0.15)vs(3.61 ±1.02),(0.13 ±0.09)vs(0.22 ±0.20),(0.96 ±0.08)vs(1.19 ±0.03),(47.65 ±3.92) vs (48.37 ±4.35) (P ＞ 0.05).The hemolytic rate of filtered group and control group were (0.08 ± 0.02) ％ vs (0.12 ± 0.03) ％.There was a significant difference.The amount of lactate(mmol/L) of filtration group and control group on the day 28 were (15.76±0.19) vs (18.73 ±0.97),while the glucose(mmo]/L) of these groups were (20.21 ±0.55) vs (18.57 ±0.46).There were significant difference in lactate and glucose.Conclusion Stored whole blood undergoes progressive changes in quality and function.Leukodepletion filtration played a positive role on hemolytic rate,lactic acid and glucose,but had no significant effect on oxygen carrying-releasing function as well as other indicators.In conclusion,the filtration has no negative effect on the whole blood storage quality,and even may be in favor of blood storage.关键词
血液保存/全血/白细胞/过滤/生理指标/生化指标Key words
blood storage/whole blood/leukocyte/filtration/physiological indexes/biochemical indexes分类
医药卫生引用本文复制引用
王红,贺曾,吴瑕,钟锐,曹晔,刘曦,何语良,袁理,刘嘉馨..滤除白细胞对全血保存质量的影响[J].中国输血杂志,2013,26(6):525-529,5.基金项目
卫生部卫生公益性行业科研专项(200902008) （200902008）
