中国实验方剂学杂志2013，Vol.19Issue(15)：117-120,4.DOI:10.11653/syfj2013150117
伸筋草药材UPLC指纹图谱的鉴别研究
Identification of Lycopodii Herba by UPLC Fingerprint
摘要
Abstract
Objective:To establish a UPLC and develop an UPLC fingerprint for the identification of Lycopodii Herba for controlling the drug quality quickly and accurately.Method:The UPLC fingerprint of ten batches of Lycopodii Herba and adulterants were determined on an Agilent ZORBAX RRHD Eclipse Plus C18 (2.1 mm × 100 mm,1.8 μm) column eluted with the mobile phase consisted of methanol-acetonitrile (1 ∶ 1)-water containing 0.05％ phosphoric acid in gradient mode and the detection wavelength was at 256 nm,the flow rate of 0.1 mL · min-1 Result:The method of HPLC fingerprints was established and the 19 mutual peaks were indicated.The similar degrees of the ten batches were between 0.923-0.985.The similarities among the Lycopodii Herba and adulterants from different sources were obtained,there were apparent difference in fingerprint.Conclusion:The method is quick and accurate.The UPLC fingerprint of Lycopodii Herba with high specificity can be used to control the quality of Lycopodii Herba.关键词
伸筋草/超高效液相色谱/指纹图谱/鉴别Key words
Lycopodii Herba/ UPLC/ fingerprint/ identification分类
医药卫生引用本文复制引用
杨烨,王祥培,孙庆文,徐锋..伸筋草药材UPLC指纹图谱的鉴别研究[J].中国实验方剂学杂志,2013,19(15):117-120,4.基金项目
贵州省优秀科技教育人才省长专项基金(黔省专合字[2011]60号) （黔省专合字[2011]60号）
