中国医科大学学报2013，Vol.42Issue(6)：501-503,520,4.
异氟醚吸入对新生大鼠海马细胞超微结构的影响
Effect of Isoflurane on Hippocampus Ultrastructure in Newborn Rats
摘要
Abstract
Objective To investigate the effects of isoflurane on mitochondria,endothelia,and nuclei of newbom rat hippocampus.Methods A total of 48 7-day old male rats were included in this study and randomly allocated into 4 groups:control 6 h group (C6),control 24 h group (C24),Isoflurane 6 h group(I6) or isoflurane 24 h group(I24),with 12 in each group.Rats in isoflurane group (I6+I24) inhaledisoflurane in oxygen and air mixture for 6 h,while rats in control group (C6+C24) inhaled vehicle for the same duration.At end of inhalation,0.3 mL to 0.5 mL blood was drawn from left ventricle for blood gas analysis from 6 rats of each group.For the rest 6 rats in each group,hippocampus was harvested by the end of 6 h(group C6 and group I6) and 24 h after inhalation separately.Slides were prepared and observed;degrees of injury were evaluated under electromicroscope by a pathologist who didn't know the details of the study.Results 6 h of isoflurane inhalation at 0.7 MAC had little effect on newbom rat by blood gas analysis (P ＞ 0.05).Isoflurane inhalation caused changes in hippocampus ultrastructure.Mitochondria and endothelia underwent balloon-like degeneration,which was more severe in I24 group than I6.Conclusion Inhalation of isoflurane causes ultrastructure changes in newborn rats hippocampus neurons.关键词
异氟醚/新生大鼠/海马/超微结构Key words
isoflurane/ newborn /rat/ hippocampus/ ultrastructure分类
医药卫生引用本文复制引用
郭瑶,祖剑宇,李妍,池晓颖,陈卫民..异氟醚吸入对新生大鼠海马细胞超微结构的影响[J].中国医科大学学报,2013,42(6):501-503,520,4.基金项目
辽宁省自然科学基金(2012225093) （2012225093）
