中国医科大学学报2013，Vol.42Issue(6)：511-514,4.
川芎嗪对大鼠脑缺血再灌注后血脑屏障的保护作用及Claudin-5表达的影响
Effects of Ligustrazine on Blood-brain Barrier and Claudin-5 Expression in Rat after Cerebral Ischemia and Reperfusion
摘要
Abstract
Objective To study the in vivo effects of ligustrazine(TMP) on blood-brain barrier (BBB) and expression of Claudin-5 in rats after brain ischemia-reperfusion (I/R).Methods The focal cerebral I/R rat model of middle cerebral artery occlusion (MCAO) was established by intraluminal suture.The change of the permeability of BBB was detected using Evans blue assay.The expression of Claudin-5 was determined by immunohischemistry assay,RT-PCR and Western blot.Results The BBB permeability was found significantly decreased at 3 h and 72 h after I/R compared with sham-operated group.The BBB permeability in ligustrazine group was found signiticantly increased compared with the group of I/R at the same time points.The expression of Claudin-5 was found significantly decreased at 3 h and 72 h after I/R group compared with sham-operated group.The expression of Claudin-5 in ligustrazine group was found significantly increased compared with the I/R group at the same testing time points.Conclusions The down-regulation of Claudin-5 is closely related to the BBB injury induced by cerebral ischemia-reperfusion.Ligustrazine contributes to the cerebral edema after reperfusion through the up-regulation of Claudin-5,recovery of function of tight junction and reducing the permeability of BBB.关键词
川芎嗪/脑缺血再灌注/Claudin-5Key words
ligustrazine/brain ischemia reperfusion/ Claudin-5分类
医药卫生引用本文复制引用
李玉芳,刘坤,倪月秋,杨智航,符文双..川芎嗪对大鼠脑缺血再灌注后血脑屏障的保护作用及Claudin-5表达的影响[J].中国医科大学学报,2013,42(6):511-514,4.基金项目
辽宁省科学技术计划(2009225009-23) （2009225009-23）
沈阳市科学技术计划(F10-205-1-21) （F10-205-1-21）
