中国药业2013，Vol.22Issue(14)：17-19,3.
高效液相色谱法测定灵芝提取物中尿苷和腺苷含量
HPLC Determination of Uridine and Adenosine Contents in Ganoderma Extracts
卢端萍 1蒋婷婷 2陈硕 1王勇1
作者信息
- 1. 福建省药品检验所,福建福州 350001
- 2. 福建省立医院,福建福州 350001
- 折叠
摘要
Abstract
Objective To establish an RP-HPLC method for the simultaneous determination of uridine and adenosine contents in Ganoderma extracts from different habitats.Methods The Agilent Zorbax SB-Aq column(250 mm÷4.6 mm,5 μm) was adopted with the mobile phase of acetonitrile-water by the gradient elution.The flow rate was 1.0 mL/min.The detection wavelength was set at 260 nm.Results The linear ranges of uridine and adenosine were (2.44-195.6) μg/mL(r =1.000 0) and (2.64-105.6) μg/mL (r =1.000 0).The average recovery rates were 102.0％ and 101.2％,RSD was 0.2％ and 1.0％ (n =6) respectively.The uridine and adenosine contents of samples had significant differences in different habitats,moreover the contents of uridine and adenosine were also different in different batches from the same habitat.Conclusion This method is simple,accurate,repeatable,which can be used to determine the contents of uridine and adenosine in Ganoderma extracts and provide the referenc basis for the quality assessment of Ganoderma extracts.关键词
高效液相色谱法/灵芝提取物/尿苷/腺苷Key words
HPLC / Ganoderma extracts/ uridine / adenosine分类
医药卫生引用本文复制引用
卢端萍,蒋婷婷,陈硕,王勇..高效液相色谱法测定灵芝提取物中尿苷和腺苷含量[J].中国药业,2013,22(14):17-19,3.
