中山大学学报（医学科学版）2013，Vol.34Issue(4)：505-512,8.
Nestin阳性细胞在小鼠心肌组织中的表达及分布特征
Expression and Distribution Characteristics of Nestin-positive Cells in Myocardial Tissue of Mouse
摘要
Abstract
[Objective] The main aim of this study was to systematically evaluate the expression patterns of the Nestin in the developing or damaged adult heart issue,and probe into whether Nestin can be as a marker of cardiac stem cell.[Methods] Nestin expression was assessed in the embryonic 13.5 d and postnatal 1 d,7 d,1 month,3 months old Nestin-GFP transgenic mouse heart tissue by fluorescence microscopy,real-time quantitative PCR and RT-PCR.Myocardial infaction model was established by ligation of left anterior descending coronary in adult Nestin-GFP mice and the Nestin expression was observed in the myocardium at 7d after injury.Then,the correlation between Nestin and other stem cell markers expression in mouse heart tissue were determined by immunofluoresent assay.[Results] In embryonic 13.5 d,the Nestin mainly expressed in the brain,spinal cord,and the retina,and also can be observed in the heart tissue.After the mouse was born,Nestin expression is gradually reduced with growth,and that was also confirmed by the RT-PCR and Q-PCR analysis.Nestin-positive cells increased significantly in myocardial infarction area compared to the normal tissue.Sca-1,c-kit,Isl-1,and Nkx2.5 were widely expressed in heart tissue,but not co-expressed with Nestin.However,in normal and injured tissue,Nestin was co-expressed with vimentin and musashi-1,neural cell marker.[Conclusion] These results indicate that Nestin expression is highly correlated with cardiac development,and the Nestin-positive myocardial cell might be arise from neural linage cells,which suggest that such cells play an important role in the growth and maintenance of the cardiogenesis and regeneration.关键词
Nestin/心肌干细胞/心肌再生Key words
Nestin/ cardiac stem cells/ myocardial regeneration分类
生物科学引用本文复制引用
吴冰原,李桂兰,姜美花,彭朝权..Nestin阳性细胞在小鼠心肌组织中的表达及分布特征[J].中山大学学报（医学科学版）,2013,34(4):505-512,8.基金项目
广东省科技计划项目(2011B031800125) （2011B031800125）
