中山大学学报（医学科学版）2013，Vol.34Issue(4)：513-516,4.
心肺复苏对心脏骤停大鼠脑线粒体功能的保护作用
Protective Effect of Cardiopulmonary Resuscitation on Brain Mitochondrial Function in a Rat Model of Cardiac Arrest
摘要
Abstract
[Objective] To investigate the protective effect of cardiopulmonary resuscitation (CPR) on brain mitochondrial function in a rat model of cardiac arrest.[Methods] Cardiac arrest rat model was established by electrical induced ventricular fibrillation.The SD rats were randomly divided into:ischemia group,in which 15 min of cardiac arrest was induced without CPR; CPR group,in which 10 min of cardiac arrest was performed followed by 5 min of CPR; and sham control group.The rats were sacrificed at the end of 15 min and hippocampal mitochondrion was isolated and extracted.Clark oxygen electrode was used to measure mitochondrial respiratory function.Mitochondrial membrane kits were used to examine the integrity of mitochondrion.Mitochondrial ultra structure was observed with transmission electron microscope.[Results] After cardiac arrest,the integrity of mitochondrial outer membrane,mitochondrial membrane potential and respiratory control rate in ischemia group and CPR group were significantly decreased than those in sham operation group (P ＜ 0.01).The result of CPR group was higher than that of ischemia group (P ＜ 0.01).There was no morphological difference between each group.[Conclusion] In the early stage of cardiac arrest,the brain mitochondrial impairment mainly manifested as functional damage rather than morphological injury.CPR could improve mitochondrial function and alleviate injury of ischemic neurons.关键词
心肺复苏/心脏骤停/大鼠/线粒体Key words
cardiopulmonary resuscitation / cardiac arrest/ rat/ mitochondrion分类
医药卫生引用本文复制引用
符岳,姜骏,许婉华,罗银秋,毛小英,谭健韶,黄子通..心肺复苏对心脏骤停大鼠脑线粒体功能的保护作用[J].中山大学学报（医学科学版）,2013,34(4):513-516,4.基金项目
国家自然科学基金资助项目(30801081) （30801081）
广东省医学科研基金(A2009179) （A2009179）
