中山大学学报（医学科学版）2013，Vol.34Issue(4)：517-520,封3,5.
血管紧张素-(1-7)对胰岛素分泌细胞株NIT-1增殖的影响
Effect of Angiotensin-(1-7) on Proliferation of Insulin-secreting Cell Line NIT-1
摘要
Abstract
[Objective] To investigate whether Angiotensin-(1-7) can affect the proliferation of mouse insulin-secreting cell line NIT-1.[Methods] NIT-1 cells were divided into different groups as follows and cell counting Kit-8 method (CCK-8) was performed to detect the proliferation of NIT-1 at different groups separately:(1) Glucose at concentration of 11.1,25.0,30.0,35.0,and 40.0 mmol/L,mannitol at concentration of 35 mmol/L for 24 h; (2) Ang-(1-7) at concentrations of 0,10-7,10-6,10-5,and 10-4 mol/L for 24 h; (3) High glucose(HG),HG+Ang-(1-7),HG+Ang-(1-7)+Mas receptor inhibitor(A-779),HG+A-779.[Results] (1)Compared with the 11.1 mmol/L group,the cell proliferation were inhibited in the 35.0 mmol/L and 40.0 mmol/L groups (1.02 ± 0.07 vs 1.21 ± 0.10; 0.90 ± 0.05 vs 1.21 ± 0.10,P ＜ 0.05).(2) No significant difference of the proliferation in N1T-1 cells was found between Ang-(1-7) treatment groups (10-7 ～ 10-4 mol/L).(3) Compared to the HG group,the proliferation of the HG+Ang-(1-7) group was higher (1.14 ± 0.07 vs 1.44 ± 0.24,P ＜ 0.05); Compared to the HG+Ang-(1-7) group,the proliferation of the HG+Ang-(1-7)+A-779 group was decreased (1.44 ± 0.24 vs 1.20 ± 0.02,P ＜ 0.05).[Conclusion] By binding to Mas receptor,Ang-(1-7) could reverse the inhibitory effect of high glucose on proliferation of NIT-1 cells.关键词
血管紧张素-(1-7)/胰岛β细胞/细胞增殖Key words
angiotensin-(1-7)/ islet β cell/ cell proliferation分类
医药卫生引用本文复制引用
王晓云,徐明彤,林秀红,宛彦,任萌,李焱,严励..血管紧张素-(1-7)对胰岛素分泌细胞株NIT-1增殖的影响[J].中山大学学报（医学科学版）,2013,34(4):517-520,封3,5.基金项目
国家自然科学基金项目(81270915),广东省科技计划(2009B030801017) （81270915）
