中山大学学报（医学科学版）2013，Vol.34Issue(4)：537-541,5.
藏红花素对耐伊马替尼慢性白血病粒细胞的促凋亡作用
Apoptotic Induction Effect and Mechanism of Crocin on Imatinib Resistant K562/Ima Cells
摘要
Abstract
[Objective] To investigate the proliferative inhibition and apoptotic induction effect of crocin on imatinib-resistant K562/Ima cells.[Methods] K562/Ima cells were seeded in the 96-well plant and treated with different concentrations of crocin for 48 h.Then CCK8 method was applied to explore the inhibition rate of crocin.The apoptosis rate of crocin on K562/Ima cells was detected by Hoechst 33258 dying and Annexin V/PI method.Caspase activity was detected after crocin treatment.[Results] Crocin could effectively inhibit K562/Ima cells growth,with a concentration dependent pattern.The apoptotic rate of K562/Ima cells was increased as the concentration of crocin was raised.Caspase-3 was activated after crocin treatment in K562/Ima cells.Caspase inhibitor Z-VAD-FMK could effectively revise this effect.[Conclusion] Crocin can significantly inhibit growth and induce apoptosis of K562/Ima cells probably via Caspase pathway.关键词
藏红花素/伊马替尼/慢性粒细胞性白血病/细胞凋亡Key words
crocin/ imatinib/ chronic myeloid leukemia/ apoptosis分类
医药卫生引用本文复制引用
张洋,郑卫东,邸玉玮,方伟,廖玉琴..藏红花素对耐伊马替尼慢性白血病粒细胞的促凋亡作用[J].中山大学学报（医学科学版）,2013,34(4):537-541,5.基金项目
广东省自然科学基金博士启动项目(2008)(8451008004001345) （2008）
