中山大学学报（医学科学版）2013，Vol.34Issue(4)：547-552,6.
自体原位肝移植术后早期胃氧化损伤及其与NF-E2相关因子2的关系
Patterns of Gastric Oxidative Damage and NF-E2-related Factor 2 Expression Alterations in SD Rats Subjected to Autologous Orthotopic Liver Transplantation
摘要
Abstract
[Objective] To investigate the dynamical changes of gastric pathology,oxidative damage and NF-E2-related factor 2 (Nrf2) expression after autologous orthotopic liver transplantation (AOLT).[Method] Twenty-four Sprague-Dawley rats were randomly divided into four groups:Sham group,and R4h,8 h and 16 h groups which were subjected to AOLT.Gastric specimens were collected to assay histopathological changes,levels of hydroxyl radical (· OH),malondialdehyde (MDA),superoxide dismutase (SOD) and catalase (CAT),and Nrf2 expression.[Results] AOLT resulted in severe gastric injury in every AOLT group,and most dramatic in group R4h.Accompanied with significant decreases in the activity of SOD and CAT,the levels of · OH and MDA increased markedly,at 4 h and 8 h after AOLT,which indicated oxidative damage in gastric tissues.The expressions of Nrf2 in gastric tissues decreases significantly at 4h after AOLT,and then restored gradually at 8 h and 16 h after AOLT.The correlation analysis showed that there were positive correlations between gastric mucosal lesion and the content of · OH and MDA,and there were negative correlations between gastric mucosal lesion and the activity of SOD and CAT (P ＜ 0.05).However the correlations between the expressions of Nrf2 and gastric mucosal lesion,the content of · OH and MDA were negative correlations,and there was positive correlation between the expressions of Nrf2 and the activity of CAT (P ＜ 0.05).[Conclusion] AOLT could resulted in severe gastric injury.The damage and restoration of gastric tissue was according with the changes of oxidation and antioxidant capacity.Up-regulation of Nrf2 expression might be beneficial to the self-healing of gastric tissue.关键词
自体原位肝移植/胃损伤/氧化损伤/抗氧化酶/NF-E2相关因子2Key words
autologous orthotopic liver transplantation/ gastric injury/ oxidative damage/ antioxidative enzyme/ NF-E2-related factor-2分类
医药卫生引用本文复制引用
葛缅,谢汉镔,黎尚荣,陆慧琼,黑子清,池信锦..自体原位肝移植术后早期胃氧化损伤及其与NF-E2相关因子2的关系[J].中山大学学报（医学科学版）,2013,34(4):547-552,6.基金项目
广东省自然科学基金(S2012010008930) （S2012010008930）
广东省中医药局课题(20121143) （20121143）
