中山大学学报（医学科学版）2013，Vol.34Issue(4)：621-626,6.
小鼠精子激活力减弱模型制备技术的初步研究
A Primary Study for Establishment of Mouse Sperm Model with Decreased Oocyte Activation Capability
摘要
Abstract
[Objective] Due to absence of sperm model with decreased oocyte activation capability,the mechanism of low rate of fertilization and the safety of artificial activation techniques applied in intracytoplasmic sperm injection are unclear.The current research focuses on a novel technique of decreasing oocyte activation capability of mouse sperms in controlled manner.[Methods] First,sperm membrane was damaged with chemical interference,mechanical sperm tail-cutting off,or combing both manipulations respectively,and then incubated for 12 h at 37 ℃ followed by intracytoplasmic sperm injection.The fertilization rates were analyzed to figure out the most effective method of decreasing the capability of sperm' s activation.Second,following the most effective treatment,the membrane-damaged sperms were incubated for different time to find out the association between the time and sperm's activation capability decreasing.Embryos' early development was also observed after intracytoplasmic sperm injection with activation capability decreased sperms.[Results] 1) Both of chemical interference and the mechanical tail-cutting off decreased fertilization rate significantly.2) When sperm tails were cut off mechanically and then incubated at 37 ℃ for 2 h,4 h,8 h or 12 h,the rates of fertilization and cleavage were decreased significantly with the increase of incubation time (P ＜ 0.01) accordingly.3) Compared with control group,the percentage of blastocyst population and hatching (P ＜ 0.001) also decreased significantly while intracytoplasmic sperm injection were applied using tail-cut sperms after incubation.[Conclusion] When sperm tails were cut off and incubated at 37 ℃,the capability of sperms to activate oocytes had significantly decreased with the increase of incubation time,and the embryo development was also disrupted.In this study,a novel technique of decreasing the sperm activation capability in controlled manner has been build up primarily.However the safety and the underlying mechanism need further explored.关键词
精子激活力/卵胞浆内单精子显微注射/卵子激活/受精率/小鼠Key words
activation capability of sperm/ intracytoplasmic sperm injection (ICSI)/ oocyte activation/ fertilization rate/ mouse分类
医药卫生引用本文复制引用
苗聪秀,燕志光,杨红梅,龙慧,柴蔚然,梁红星,于莎,匡延平,吕祁峰..小鼠精子激活力减弱模型制备技术的初步研究[J].中山大学学报（医学科学版）,2013,34(4):621-626,6.基金项目
国家自然科学基金(31071275,81270749) （31071275,81270749）
国家自然科学基金青年项目(31101070) （31101070）
上海市科委项目(09411962900,11411950105,12ZR1416600) （09411962900,11411950105,12ZR1416600）
