南方农业学报2013，Vol.44Issue(7)：1069-1075,7.DOI:10.3969/j:issn.2095-1191.2013.7.1069
参薯ADP-ribosylation factor(ARF)基因的生物信息学分析
Bioinformatics analysis of ADP-ribosylation factor (ARF) gene from Dioscorea alata L.
摘要
Abstract
[Objective]The bioinformatics analysis of 11 plants including Dioscorea alata L.was conducted to provide references for further research on structure and function of plant ARF gene.[Method]The cloning and sequence analysis of homologous fragments of ARF gene from Dioscorea alata L.were performed and predicted by the tools of bioinformatics in the following aspects:the composition of nucleotide sequences,amino acid sequences,components,hydrophobicity or hydrophily,secondary and tertiary structure of protein,signal peptide,transmembrane structure and leader peptide.[Result]The homologous fragment of ARF gene from Dioscorea alata L.was 1200 bp.Phylogenetic analysis showed that ARF amino acid sequence of the 11 plants was classified into two classes,Ⅰ and Ⅱ,which included five subtribes of A,B,C,D and E.Dioscorea alata L.ARF 1 and Medicago truncatula formed a subtribe.Dioscorea alata L.ARF 2 and Hyacinthus orientalis was a subtribe.GTP/Mg2+ binding site,G2 and G3 conservative domain existed in the amino acid sequence of Dioscorea alata L.ARF.There was the same effector domain Switch 1 and Switch 2.The structure of ARF protein from Dioscorea alata L.was mainly random coil.Its third-level structure was not steady.ARF protein was fat soluble protein with hydrophobicity and obvious membrane spaning domain,but there was no signal peptide.The targeting sequence of ARF gene had no definite position,which was predicted to be at level three.[Conclusion]The particularity of ARF protein from Dioscorea a/ata L.could serve as a foundation for material transport and involvement in signal transduction functions.关键词
参薯/ARF基因/生物信息学/聚类分析/蛋白结构Key words
Dioscorea alata L./ ADP-ribosylation factor(ARF) / bioinformatics/ cluster analysis/ protein structure分类
农业科技引用本文复制引用
高洪昌,黄小龙,陈银华,许云,吴文嫱,谢俊,黄东益..参薯ADP-ribosylation factor(ARF)基因的生物信息学分析[J].南方农业学报,2013,44(7):1069-1075,7.基金项目
高等学校博士学科点专项科研基金项目(20114601110001) （20114601110001）
海南省自然科学基金项目(310024) （310024）
